内复制的鼠滋养层细胞功能特征的方案。

Protocol for functional characterization of endoreduplicated murine trophoblast cells.

机构信息

Division of Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India.

出版信息

STAR Protoc. 2022 Jul 20;3(3):101573. doi: 10.1016/j.xpro.2022.101573. eCollection 2022 Sep 16.

DOI:10.1016/j.xpro.2022.101573

PMID:35880131

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9307567/

Abstract

Murine trophoblast stem cells (TSCs) have shaped placental research by providing resources for investigating trophoblast subtype specialization. Trophoblast giant cells (TGCs) are large polyploid cells, which undergo repetitive rounds of DNA replication without intervening mitosis by a process called endoreduplication. Endocrine and paracrine functions of TGCs aid in maternal adaptations to pregnancy. Here, we describe a protocol for differentiation of murine TSCs to TGCs together with the genotypic as well as phenotypic characterization of the endoreduplicated TGCs. For complete details on the use and execution of this protocol, please refer to Basak and Ain (2022).

摘要

鼠滋养层干细胞（TSC）通过为研究滋养层亚型特化提供资源，为胎盘研究提供了帮助。滋养层巨细胞（TGC）是大的多倍体细胞，通过一种称为核内复制的过程，在没有有丝分裂介入的情况下，经历重复的 DNA 复制。TGC 的内分泌和旁分泌功能有助于母体适应妊娠。在这里，我们描述了一种将鼠 TSC 分化为 TGC 的方案，以及对核内复制的 TGC 的基因型和表型特征进行了描述。有关该方案使用和执行的完整详细信息，请参考 Basak 和 Ain（2022 年）。