State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
STAR Protoc. 2023 Mar 17;4(1):102092. doi: 10.1016/j.xpro.2023.102092. Epub 2023 Feb 3.
Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. The iTSCs show typical TSC properties and have the potential to differentiate into syncytiotrophoblast cells (STCs) and trophoblast giant cells (TGCs). This cell fate transition provides a general platform to robustly investigate the mechanisms underlying TE specification. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022)..
从内细胞团衍生的胚胎干细胞（ESCs）中自然转化滋养外胚层（TE）衍生的滋养层干细胞（TSCs）是困难的。在这里，我们通过体外 Tet-on 系统介绍了一种从 ESCs 生成诱导性 TSCs（iTSCs）的可靠且可重复的方案。iTSCs 表现出典型的 TSC 特性，并具有分化为合体滋养层细胞（STCs）和滋养层巨细胞（TGCs）的潜力。这种细胞命运转变为深入研究 TE 特化的机制提供了一个通用平台。有关此方案使用和执行的完整详细信息，请参阅 Zhang 等人（2022 年）。
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