Xu Yi-Qian, Cui Xiang, Liu Kun, Song Ling, Wang Shu-Ya, Li Wang, Zhu Bing, Gao Xin-Yan
Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Research Center for Aerosol Inhalation, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700.
Zhen Ci Yan Jiu. 2022 Jul 25;47(7):580-6. doi: 10.13702/j.1000-0607.20211299.
To observe the effect of electroacupuncture (EA) and pre-EA at"Taiyuan"(LU9) on histopathological changes, pulmonary ventilation function, pneodynamics, and contents of TNF-α, IL-1β and IL-10 in the bronchoalveolar lavage fluid in rats with acute lung injury (ALI), in order to explore their differences of therapeutic effects.
A total of 92 male SD rats were randomly divided into control, model, routine EA and pre-EA groups, with 23 rats in each group. The ALI rat model was established by instillation of 0.5% lipopolysaccharide solution (5 mg/kg) intratracheally. EA (3 mA, 10 Hz) was applied to bilateral LU9 for 20 min, once 5 h after modeling for rats of the routine EA group, and once on the 1, 3 and 5 day respectively before modeling for rats of the pre-EA group. The pulmonary ventilation functions, including dynamic lung compliance (Cdyn), tidal volume (TV)，minute ventilation volume (MV), lung resistance, peak inspiratory flow (PIF), peak expiratory flow (PEF), were measured by using a small animal lung function detection system. The electromyogram (EMG) of the diaphragm muscle was recorded for observing changes of the total integral electromyogram(iEMG), single iEMG and respiration rate. Pathological changes of the lung tissue were scored after H.E. staining. The contents of TNF-α, IL-1β and IL-10 in the bronchoalveolar lavage fluid were determined by enzyme linked immunosorbent assay.
Compared with the control group, the Cdyn, TV, MV and PIF consi-derably decreased (<0.000 1), and the lung resistance, total iEMG of diaphragm muscle, lung injury score, and contents of TNF-α and IL-1β were significantly increased (<0.05, <0.001, <0.01) in the model group. In comparison with the model group, the TV, MV and PIF in both routine EA and pre-EA groups,and the Cdyn, total iEMG and single iEMG in the routine EA group, as well as the IL-10 content in the pre-EA group were significantly increased (<0.01, <0.001, <0.05), whereas the lung injury score, and contents of TNF-α and IL-1β were significantly decreased in the pre-EA group (<0.05).
Both pre-EA and routine EA at LU9 can improve the lung function of ALI model rats. Pre-electroacupuncture is related to local immunoregulation, while electroacupuncture is mainly related to the improvement of pulmonary ventilation function and respiratory motility.
观察电针及“太渊”（LU9）预电针对急性肺损伤（ALI）大鼠组织病理学变化、肺通气功能、肺动力学及支气管肺泡灌洗液中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-10（IL-10）含量的影响，以探讨两者治疗效果的差异。
将92只雄性SD大鼠随机分为对照组、模型组、常规电针组和预电针组，每组23只。通过气管内滴注0.5%脂多糖溶液（5 mg/kg）建立ALI大鼠模型。常规电针组大鼠于造模后5 h对双侧LU9施加电针（3 mA，10 Hz）20 min，预电针组大鼠分别于造模前第1、3、5天对双侧LU9施加电针（3 mA，10 Hz）20 min。采用小动物肺功能检测系统测量肺通气功能，包括动态肺顺应性（Cdyn）、潮气量（TV）、分钟通气量（MV）、肺阻力、吸气峰流速（PIF）、呼气峰流速（PEF）。记录膈肌肌电图（EMG），观察总积分肌电图（iEMG）、单次iEMG及呼吸频率的变化。HE染色后对肺组织病理变化进行评分。采用酶联免疫吸附测定法测定支气管肺泡灌洗液中TNF-α、IL-1β和IL-10的含量。
与对照组比较，模型组Cdyn、TV、MV和PIF显著降低（<0.000 1），肺阻力、膈肌总iEMG、肺损伤评分以及TNF-α和IL-1β含量显著升高（<0.05，<0.001，<0.01）。与模型组比较，常规电针组和预电针组的TV、MV和PIF，常规电针组的Cdyn、总iEMG和单次iEMG，以及预电针组的IL-10含量均显著升高（<0.01，<0.001，<0.05），而预电针组的肺损伤评分以及TNF-α和IL-1β含量显著降低（<0.05）。
LU9预电针和常规电针均可改善ALI模型大鼠的肺功能。预电针与局部免疫调节有关；电针主要与改善肺通气功能及呼吸动力有关。
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