Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.
Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.
Acupunct Med. 2023 Jun;41(3):175-182. doi: 10.1177/09645284221118588. Epub 2022 Aug 30.
Macrophage polarization toward the M2 phenotype may attenuate inflammation and have a therapeutic effect in acute lung injury (ALI).
To investigate the role of electroacupuncture (EA) pretreatment on the inflammatory response and macrophage polarization in a septic rat model of lipopolysaccharide (LPS)-induced ALI.
Male Sprague Dawley rats (n = 24) were randomly divided into three groups (n = 8 each): control (Ctrl), ALI (LPS) and pre-EA (LPS + EA pretreatment). ALI and pre-EA rats were injected with LPS via the caudal vein. Pulmonary edema was assessed by left upper pulmonary lobe wet-to-dry (W/D) ratios. Lung injury scores were obtained from paraffin-embedded and hematoxylin and eosin-stained sections of the left lower pulmonary lobe. Inflammatory activation was quantified using serum tumor necrosis factor (TNF)-α, interleukin (IL)-1β, transforming growth factor (TGF)-β and IL-10 levels measured by enzyme linked immunosorbent assay (ELISA). Macrophage phenotype was determined by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting.
Mean lung W/D ratio was significantly lower and serum IL-1β levels were decreased in pre-EA rats compared to ALI rats (P < 0.05). TNF-α mRNA expression was decreased and mannose receptor (MR) and Arg1 mRNA expression was increased in the lung tissues of pre-EA rats compared to ALI rats (P < 0.01). Arg1 protein expression was similarly increased in the lung tissues of pre-EA rats compared to ALI rats (P < 0.05).
EA pretreatment may play a protective role by promoting macrophage polarization to the M2 phenotype in a septic rat model of LPS-induced ALI.
巨噬细胞向 M2 表型极化可能会减轻炎症，并对急性肺损伤 (ALI) 产生治疗作用。
探讨电针预处理在脂多糖 (LPS) 诱导的 ALI 脓毒症大鼠模型中对炎症反应和巨噬细胞极化的作用。
雄性 Sprague Dawley 大鼠 (n = 24) 随机分为三组 (n = 8 只/组)：对照组 (Ctrl)、ALI 组 (LPS) 和电针预处理组 (LPS + EA 预处理)。ALI 和电针预处理组大鼠通过尾静脉注射 LPS。通过左肺上叶湿重/干重 (W/D) 比值评估肺水肿。从左下肺叶石蜡包埋和苏木精-伊红染色切片中获得肺损伤评分。通过酶联免疫吸附试验 (ELISA) 测量血清肿瘤坏死因子 (TNF)-α、白细胞介素 (IL)-1β、转化生长因子 (TGF)-β 和 IL-10 水平来量化炎症激活。通过实时定量聚合酶链反应 (RT-qPCR) 和 Western blot 测定巨噬细胞表型。
与 ALI 组相比，电针预处理组大鼠平均肺 W/D 比值显著降低，血清 IL-1β 水平降低 (P < 0.05)。与 ALI 组相比，电针预处理组大鼠肺组织 TNF-α mRNA 表达降低，甘露糖受体 (MR) 和 Arg1 mRNA 表达增加 (P < 0.01)。与 ALI 组相比，电针预处理组大鼠肺组织 Arg1 蛋白表达也增加 (P < 0.05)。
电针预处理可能通过促进巨噬细胞向 LPS 诱导的 ALI 脓毒症大鼠模型中的 M2 表型极化发挥保护作用。
