Liven E
Acta Vet Scand. 1978;19(2):184-91. doi: 10.1186/BF03547624.
The activities of proteinases, lipases, amylases and the activities of proteinase inhibitors, as well as the numbers of Escherichia coli in the contents from the small intestine were examined for pigs suffering from colienterotoxaemia and for healthy pigs. Enzyme activities were determined using an agar diffusion test. Compared with healthy animals the activities of proteinases and amylases in diseased animals were reduced while lipases showed increased activity. In pathologically changed contents showing large numbers of E. coli, proteinases could not be demonstrated; however, proteinase inhibitors were found in these contents. In healthy animals, proteinase inhibitors were not demonstrated in ingesta-con-taining contents. In diseased animals, E. coli were found in large numbers in all parts of the small intestine. In healthy animals, E. coli was demonstrated especially in the posterior part of the small intestine and regularly in small numbers. The possible influence of digestive enzymes, especially proteinases and their inhibitors, on enterotoxins from E. coli is discussed.
对患有大肠杆菌肠毒血症的猪和健康猪的小肠内容物中的蛋白酶、脂肪酶、淀粉酶活性以及蛋白酶抑制剂活性，还有大肠杆菌数量进行了检测。酶活性通过琼脂扩散试验测定。与健康动物相比，患病动物体内蛋白酶和淀粉酶的活性降低，而脂肪酶活性增加。在显示大量大肠杆菌的病理变化内容物中，无法检测到蛋白酶；然而，在这些内容物中发现了蛋白酶抑制剂。在健康动物中，含有食入物的内容物中未检测到蛋白酶抑制剂。在患病动物中，小肠各部位均发现大量大肠杆菌。在健康动物中，大肠杆菌尤其在小肠后部被检测到，且数量通常较少。文中讨论了消化酶，尤其是蛋白酶及其抑制剂对大肠杆菌肠毒素的可能影响。
