建立和维持恶性疟原虫无性血期的体外培养。

Establishing and Maintaining In Vitro Cultures of Asexual Blood Stages of Plasmodium falciparum.

机构信息

Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.

出版信息

Methods Mol Biol. 2022;2470:37-49. doi: 10.1007/978-1-0716-2189-9_5.

DOI:10.1007/978-1-0716-2189-9_5

PMID:35881337

Abstract

In vitro culture of asexual blood stages of Plasmodium falciparum is essential to study the parasite biology, and several aspects need to be addressed to successfully cultivate the parasites, including the requirements for red blood cells and specific nutrients. Since Trager and Jensen established the technique in 1976, some modifications have been introduced to improve the growth rate and yield. In brief, the method is based on the use of human red blood cells suspended in RPMI-1640 culture medium supplemented with a source of lipids and maintained at 37 °C in a low-oxygen atmosphere. In this protocol, a description of thawing, culturing, and cryopreservation of asexual blood stages of P. falciparum is presented.

摘要

体外培养疟原虫无性血阶段对于研究寄生虫生物学至关重要，需要解决多个方面的问题才能成功培养寄生虫，包括对红细胞和特定营养物质的需求。自 1976 年 Trager 和 Jensen 建立该技术以来，已经进行了一些改进以提高生长速度和产量。简而言之，该方法基于使用悬浮在 RPMI-1640 培养基中的人红细胞，该培养基中添加了脂质来源，并在 37°C 低氧环境中保存。在本方案中，介绍了疟原虫无性血阶段的解冻、培养和冷冻保存。