Maier Alexander G, Rug Melanie
Research School of Biology, The Australian National University, Canberra, ACT, Australia.
Methods Mol Biol. 2013;923:3-15. doi: 10.1007/978-1-62703-026-7_1.
The in vitro cultivation of Plasmodium falciparum is absolutely essential for the molecular dissection of parasite biology and still poses several challenges. The dependence on, and interaction with host red blood cells, the tightly regulated stage-specific expression of proteins, and the parasite peculiar demands on nutrients and gaseous environments are only a few aspects that need to be addressed to successfully cultivate P. falciparum in vitro. In this chapter, we present techniques for normal maintenance of the erythrocytic stages of P. falciparum cultures, their synchronization and the generation of clonal cell lines.
恶性疟原虫的体外培养对于寄生虫生物学的分子剖析绝对至关重要，并且仍然面临若干挑战。对宿主红细胞的依赖及其与之的相互作用、蛋白质严格调控的阶段特异性表达，以及寄生虫对营养物质和气态环境的特殊需求，只是成功在体外培养恶性疟原虫需要解决的几个方面。在本章中，我们介绍了恶性疟原虫培养物红细胞阶段的常规维持、同步化以及克隆细胞系生成的技术。
