流式细胞术分析α-巨球蛋白与疟原虫感染红细胞的非免疫性 IgM 结合。

Analysis by Flow Cytometry of α-Macroglobulin and Nonimmune IgM-Binding to Plasmodium falciparum-Infected Erythrocytes.

机构信息

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Centre for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark.

Department of Infectious Diseases, Centre for Medical Parasitology, Rigshospitalet, Copenhagen, Denmark.

出版信息

Methods Mol Biol. 2022;2470:435-444. doi: 10.1007/978-1-0716-2189-9_32.

DOI:10.1007/978-1-0716-2189-9_32

PMID:35881364

Abstract

Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α-macroglobulin (αM). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and αM-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.

摘要

恶性疟原虫红细胞膜蛋白 1（PfEMP1）家族的几个成员可以结合人血清蛋白，如 IgM 和 α-巨球蛋白（αM）。这种结合似乎通过提高 PfEMP1 介导与红细胞受体结合的亲和力和/或通过掩盖 PfEMP1 中的抗体表位，在发病机制和免疫逃避中发挥作用。在本方案中，我们描述了一种基于流式细胞术的方法，用于评估完整和未固定的成熟期 IEs 中 IgM 和 αM 的结合。该方法可用于实验室克隆和现场分离株。