Grady Sarah L, Sebeck Natalie M, Theodore Mellisa, Meidenbauer Karen L
Applied Physics Laboratory, Johns Hopkins University, Laurel, MD 20723, USA.
Animals (Basel). 2022 Jul 17;12(14):1823. doi: 10.3390/ani12141823.
Given the increased deployment of working dogs to settings with pathogenic biological agents, a safe, effective, and logistically feasible surface decontamination protocol is essential to protect both the animals and their human handlers. Our group previously found that superficial contamination on surfaces relevant to the working dog community, including leashes and toys, could be significantly reduced using a standardized wiping protocol with various cleansing products. To expand upon this work, we analyzed the ability of this protocol to decontaminate surface-deposited bovine coronavirus, which was used as a BSL2 surrogate for SARS-CoV-2. Unsurprisingly, the physical characteristics of a given surface, including porosity and texture, had a significant effect on the ability to recover viable virus remaining on the surface post treatment. After correcting for these differences, however, wiping with 70% isopropyl alcohol (IPA) and 0.5% chlorhexidine performed best, reducing viral titers by >3 log on plastic bumper toys and nylon collars, and by >2 log on rubber toys and tennis balls. Leather leashes and Velcro proved more difficult to decontaminate, but both still showed significant loss of viral contamination following wiping with IPA or chlorhexidine. This work (i) validates the utility of a simple protocol for the neutralization of viruses on several surfaces, (ii) identifies materials that are more difficult to decontaminate, which should, thus, be considered for removal from field use, and (iii) highlights the need for further development of protocols testing porous or textured surfaces.
鉴于工作犬越来越多地被部署到存在致病性生物制剂的环境中，一套安全、有效且在后勤方面可行的表面去污方案对于保护动物及其人类 handlers 至关重要。我们的团队此前发现，使用标准化擦拭方案搭配各种清洁产品，可以显著减少与工作犬群体相关表面（包括皮带和玩具）的表面污染。为了拓展这项工作，我们分析了该方案对表面沉积的牛冠状病毒进行去污的能力，牛冠状病毒被用作 SARS-CoV-2 的 BSL2 替代物。不出所料，给定表面的物理特性，包括孔隙率和质地，对处理后留在表面的活病毒的回收能力有显著影响。然而，在校正这些差异后，用 70%异丙醇（IPA）和 0.5%洗必泰擦拭效果最佳，在塑料保险杠玩具和尼龙项圈上病毒滴度降低>3 个对数，在橡胶玩具和网球上降低>2 个对数。皮革皮带和魔术贴证明更难去污，但在用 IPA 或洗必泰擦拭后，两者的病毒污染仍有显著减少。这项工作（i）验证了一种简单方案对多种表面上病毒的中和作用，（ii）识别出更难去污的材料，因此应考虑将其从现场使用中移除，（iii）强调了进一步开发测试多孔或有纹理表面方案的必要性。
