发展一种多酶法，用阿魏酸修饰生物聚合物。
Development of a Multi-Enzymatic Approach for the Modification of Biopolymers with Ferulic Acid.
机构信息
Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece.
Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece.
出版信息
Biomolecules. 2022 Jul 17;12(7):992. doi: 10.3390/biom12070992.
A series of polymers, including chitosan (CS), carboxymethylcellulose (CMC) and a chitosan-gelatin (CS-GEL) hybrid polymer, were functionalized with ferulic acid (FA) derived from the enzymatic treatment of arabinoxylan through the synergistic action of two enzymes, namely, xylanase and feruloyl esterase. Subsequently, the ferulic acid served as the substrate for laccase from (AbL) in order to enzymatically functionalize the above-mentioned polymers. The successful grafting of the oxidized ferulic acid products onto the different polymers was confirmed through ultraviolet-visible (UV-Vis) spectroscopy, attenuated total reflectance (ATR) spectroscopy, scanning electron microscopy (SEM) and nuclear magnetic resonance (NMR) spectroscopy. Additionally, an enhancement of the antioxidant properties of the functionalized polymers was observed according to the DDPH and ABTS protocols. Finally, the modified polymers exhibited strong antimicrobial activity against bacterial populations of BL21DE3 strain, suggesting their potential application in pharmaceutical, cosmeceutical and food industries.
一系列聚合物，包括壳聚糖（CS）、羧甲基纤维素（CMC）和壳聚糖-明胶（CS-GEL）杂化聚合物，通过两种酶，即木聚糖酶和阿魏酸酯酶的协同作用，用来源于酶处理阿拉伯木聚糖的阿魏酸（FA）进行了功能化。随后，阿魏酸作为漆酶（来自 ）的底物，以酶促方式对上述聚合物进行功能化。通过紫外-可见（UV-Vis）光谱、衰减全反射（ATR）光谱、扫描电子显微镜（SEM）和核磁共振（NMR）光谱证实了氧化阿魏酸产物成功接枝到不同聚合物上。此外，根据 DDPH 和 ABTS 方案观察到功能化聚合物抗氧化性能的增强。最后，改性聚合物对 BL21DE3 菌株的细菌种群表现出强烈的抗菌活性，表明它们在制药、化妆品和食品工业中的潜在应用。
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