通过 CRISPR-Cas9 核酸酶工程提高基因编辑特异性的最新进展。
Recent Advances in Improving Gene-Editing Specificity through CRISPR-Cas9 Nuclease Engineering.
机构信息
Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
出版信息
Cells. 2022 Jul 13;11(14):2186. doi: 10.3390/cells11142186.
CRISPR-Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR-Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.
CRISPR-Cas9 是一种先进的可编程基因组编辑工具，广泛应用于许多领域。为了在临床医学中安全地进行治疗应用，必须显著降低其脱靶效应。近年来，已经进行了广泛的研究，以使用不同策略来提高最流行的 CRISPR-Cas9 核酸酶的基因编辑特异性。在这篇综述中，我们总结和讨论了这些策略和成就，主要重点是通过 Cas9 蛋白工程来提高基因编辑特异性。
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