Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Animal Functional Genomics Group, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Genes (Basel). 2022 Jun 22;13(7):1114. doi: 10.3390/genes13071114.
Genetic information is loaded on chromatin, which involves DNA sequence arrangement and the epigenetic landscape. The epigenetic information including DNA methylation, nucleosome positioning, histone modification, 3D chromatin conformation, and so on, has a crucial impact on gene transcriptional regulation. Out of them, nucleosomes, as basal chromatin structural units, play an important central role in epigenetic code. With the discovery of nucleosomes, various nucleosome-level technologies have been developed and applied, pushing epigenetics to a new climax. As the underlying methodology, next-generation sequencing technology has emerged and allowed scientists to understand the epigenetic landscape at a genome-wide level. Combining with NGS, nucleosome-omics (or nucleosomics) provides a fresh perspective on the epigenetic code and 3D genome landscape. Here, we summarized and discussed research progress in technology development and application of nucleosome-omics. We foresee the future directions of epigenetic development at the nucleosome level.
遗传信息加载在染色质上，涉及 DNA 序列排列和表观遗传景观。表观遗传信息包括 DNA 甲基化、核小体定位、组蛋白修饰、三维染色质构象等，对基因转录调控有至关重要的影响。其中，核小体作为基本染色质结构单元，在表观遗传密码中起着重要的核心作用。随着核小体的发现，各种核小体水平的技术已经被开发并应用，将表观遗传学推向了一个新的高潮。作为基础方法学，下一代测序技术已经出现，并使科学家能够在全基因组水平上理解表观遗传景观。结合 NGS，核小体组学（或核组学）为理解表观遗传密码和三维基因组景观提供了新的视角。在这里，我们总结和讨论了核小体组学技术发展和应用的研究进展。我们预见了核小体水平上的表观遗传学发展的未来方向。
