Research Unit, Hospital Universitario Dr. Negrín, 35019 Las Palmas de Gran Canaria, Spain.
Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), 35019 Las Palmas de Gran Canaria, Spain.
Int J Environ Res Public Health. 2022 Jul 16;19(14):8672. doi: 10.3390/ijerph19148672.
(1) Background: Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) continues to cause profound health, economic, and social problems worldwide. The management and disinfection of materials used daily in health centers and common working environments have prompted concerns about the control of coronavirus disease 2019 (COVID-19) infection risk. Ozone is a powerful oxidizing agent that has been widely used in disinfection processes for decades. The aim of this study was to assess the optimal conditions of ozone treatment for the elimination of heat-inactivated SARS-CoV-2 from office supplies (personal computer monitors, keyboards, and computer mice) and clinical equipment (continuous positive airway pressure tubes and personal protective equipment) that are difficult to clean. (2) Methods: The office supplies and clinical equipment were contaminated in an area of 1 cm with 1 × 10 viral units of a heat-inactivated SARS-CoV-2 strain, then treated with ozone using two different ozone devices: a specifically designed ozonation chamber (for low-medium ozone concentrations over large volumes) and a clinical ozone generator (for high ozone concentrations over small volumes). SARS-CoV-2 gene detection was carried out using quantitative real-time polymerase chain reaction (RT-qPCR). (3) Results: At high ozone concentrations over small surfaces, the ozone eliminated SARS-CoV-2 RNA in short time periods-i.e., 10 min (at 4000 ppm) or less. The optimum ozone concentration over large volumes was 90 ppm for 120 min in ambient conditions (24 °C and 60-75% relative humidity). (4) Conclusions: This study showed that the appropriate ozone concentration and exposure time eliminated heat-inactivated SARS-CoV-2 RNA from the surfaces of different widely used clinical and office supplies, decreasing their risk of transmission, and improving their reutilization. Ozone may provide an additional tool to control the spread of the COVID-19 pandemic.
（1）背景：严重急性呼吸系统综合症冠状病毒 2 型（SARS-CoV-2）继续在全球范围内造成严重的健康、经济和社会问题。对卫生中心和常见工作环境中日常使用材料的管理和消毒，引发了对控制 2019 年冠状病毒病（COVID-19）感染风险的关注。臭氧是一种强大的氧化剂，已在消毒过程中使用了数十年。本研究旨在评估臭氧处理的最佳条件，以消除难以清洁的办公用品（个人电脑显示器、键盘和计算机鼠标）和临床设备（持续气道正压管和个人防护设备）上热灭活的 SARS-CoV-2。
（2）方法：在 1 平方厘米的区域内用 1×10 个病毒单位的热灭活 SARS-CoV-2 株污染办公用品和临床设备，然后使用两种不同的臭氧设备进行臭氧处理：一个专门设计的臭氧室（用于大容量的中低浓度臭氧）和一个临床臭氧发生器（用于小体积的高浓度臭氧）。使用实时聚合酶链反应（RT-qPCR）定量检测 SARS-CoV-2 基因。
（3）结果：在小面积的高臭氧浓度下，臭氧在短时间内消除了 SARS-CoV-2 RNA，即 10 分钟（在 4000 ppm 或更低浓度下）。在环境条件（24°C 和 60-75%相对湿度）下，体积较大时的最佳臭氧浓度为 90 ppm，持续 120 分钟。
（4）结论：本研究表明，适当的臭氧浓度和暴露时间可以从不同常用临床和办公用品的表面消除热灭活的 SARS-CoV-2 RNA，降低其传播风险，提高其再利用率。臭氧可能为控制 COVID-19 大流行的传播提供额外的工具。
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