Jacobsen C, Kenney J M, Kirz J, Rosser R J, Cinotti F, Rarback H, Pine J
Phys Med Biol. 1987 Apr;32(4):431-7. doi: 10.1088/0031-9155/32/4/002.
A scanning soft x-ray microscope has been developed that uses synchrotron radiation focused by a Fresnel zone plate to form a submicron beamspot on the specimen. Transmitted x-rays are detected and used to form a quantitative map of specimen absorptivity. Applications of the instrument to the imaging of whole wet cells and to the mapping of calcium in sections of bone are presented, with a resolution of 300 nm and an elemental sensitivity of 2 micrograms cm-2.
一种扫描软X射线显微镜已被研制出来,它利用由菲涅耳波带片聚焦的同步辐射在样品上形成亚微米束斑。透射的X射线被检测到并用于形成样品吸收率的定量图谱。介绍了该仪器在完整湿细胞成像和骨切片中钙的图谱绘制方面的应用,分辨率为300纳米,元素灵敏度为2微克/平方厘米。