Ding Zhenyu, Peng Qian, Zuo Jun, Wang Yuehong, Zhou Hongbo, Tang Zhangui
Xiangya Stomatological Hospital, Central South University, Changsha 410008, China.
Xiangya School of Stomatology, Central South University, Changsha 410008, China.
Materials (Basel). 2022 Jul 18;15(14):4985. doi: 10.3390/ma15144985.
The boronized Ti6Al4V/HA composite is deemed to be an important biomaterial because of its potential remarkable mechanical and biological properties. This paper reports the osteogenesis performance of the boronized Ti6Al4V/HA composite, which was prepared by microwave sintering of powders of Ti6Al4V, hydroxyapatite (HA), and TiB in high-purity Ar gas at 1050 °C for 30 min, as dental implant based on both cell experiments in vitro and animal experiments in vivo. The comparison between the boronized Ti6Al4V/HA composite and Ti, Ti6Al4V, and boronized Ti6Al4V in the terms of adhesion, proliferation, alkaline phosphate (ALP) activity, and mineralization of MG-63 cells on their surfaces confirmed that the composite exhibited the best inductive osteogenesis potential. It exerted a more significant effect on promoting the early osteogenic differentiation of osteoblasts and exhibited the maximum optical density (OD) value in the MTT assay and the highest levels of ALP activity and mineralization ability, primarily ascribed to its bioactive HA component, porous structure, and relatively rough micro-morphology. The in vivo study in rabbits based on the micro-computed tomography (micro-CT) analysis, histological and histomorphometric evaluation, and biomechanical testing further confirmed that the boronized Ti6Al4V/HA composite had the highest new bone formation potential and the best osseointegration property after implantation for up to 12 weeks, mainly revealed by the measured values of bone volume fraction, bone implant contact, and maximum push-out force which, for example, reached 48.64%, 61%, and 150.3 ± 6.07 N at the 12th week. Owing to these inspiring features, it can serve as a highly promising dental implant.
渗硼Ti6Al4V/羟基磷灰石（HA）复合材料因其潜在的卓越机械性能和生物学性能而被视为一种重要的生物材料。本文报道了渗硼Ti6Al4V/HA复合材料的成骨性能，该复合材料是通过在高纯氩气中于1050℃下对Ti6Al4V、羟基磷灰石（HA）和TiB粉末进行微波烧结30分钟制备而成，作为牙科植入物，基于体外细胞实验和体内动物实验进行研究。渗硼Ti6Al4V/HA复合材料与Ti、Ti6Al4V和渗硼Ti6Al4V在MG-63细胞在其表面的黏附、增殖、碱性磷酸酶（ALP）活性和矿化方面的比较证实，该复合材料表现出最佳的诱导成骨潜力。它对促进成骨细胞的早期成骨分化具有更显著的作用，在MTT试验中表现出最大光密度（OD）值以及最高水平的ALP活性和矿化能力，这主要归因于其生物活性HA成分、多孔结构和相对粗糙的微观形态。基于微计算机断层扫描（micro-CT）分析、组织学和组织形态计量学评估以及生物力学测试的兔体内研究进一步证实，渗硼Ti6Al4V/HA复合材料在植入长达12周后具有最高的新骨形成潜力和最佳的骨整合性能，主要通过骨体积分数、骨植入物接触和最大推出力的测量值得以体现，例如，在第12周时分别达到48.64%、61%和150.3±6.07 N。由于这些令人鼓舞的特性，它可作为一种极具前景的牙科植入物。
