用于牙种植体的钛-羟基磷灰石功能梯度材料的体内和体外分析。
In Vivo and In Vitro Analyses of Titanium-Hydroxyapatite Functionally Graded Material for Dental Implants.
机构信息
The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, China.
Key Laboratory of Oral Biomedical Research of Zhejiang Province, China.
出版信息
Biomed Res Int. 2021 Apr 30;2021:8859945. doi: 10.1155/2021/8859945. eCollection 2021.
PURPOSE
The stress shielding effect caused due to the mechanical mismatch between the solid titanium and the surrounding bone tissue warrants the utilization of a mechanically and biologically compatible material such as the titanium-hydroxyapatite (Ti-HA) functionally graded material (FGM) for dental implants. This study is aimed at fabricating a Ti-HA FGM with superior mechanical and biological properties for dental implantation.
MATERIALS AND METHODS
We fabricated a Ti-HA FGM with different Ti volume fractions (VFs) using HA and Ti powders. Ti-HA was characterized by studying its mechanical properties. Cytotoxicity was examined using a Cell Counting Kit-8 assay and an LDH cell cytotoxicity assay. Scanning electron microscopy was performed on an XL30 environmental scanning electron microscope (ESEM). Alkaline phosphatase (ALP) and transforming growth factor (TGF-1) expressions were quantitatively monitored using enzyme-linked immunosorbent assay (ELISA) kits. The expressions of - receptors and ALP genes were measured using real-time polymerase chain reaction. The Ti-HA FGM dental implants were placed in beagle dogs. Microcomputed tomography (CT) and hard tissue slices were performed to evaluate the bone-implant contact (BIC) and bone volume over total volume (BV/TV).
RESULTS
The density and mechanical properties of the Ti-HA exhibited various graded distributions corresponding to VF. Based on the results of the Cell Counting Kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays, the difference in cytotoxicity between the two groups was statistically nonsignificant ( = 0.11). The ALP and TGF-1 levels were slightly upregulated. The transcript levels of ALP and TGF-RI were higher in the Ti-HA groups than in the Ti group at 7 days, whereas the transcript levels of TGF-RII exhibited no obvious increase. The BIC did not exhibit significant differences between the Ti and Ti-HA FGM groups ( = 0.0504). BV/TV showed the Ti-HA FGM group had better osteogenesis ( = 0.04).
CONCLUSION
Ti-HA FGM contributes to the osteogenesis of dental implants in vivo and in vitro.
目的
由于固体钛与周围骨组织之间的机械不匹配而导致的应力屏蔽效应，需要使用机械和生物相容性材料，如钛-羟基磷灰石（Ti-HA）功能梯度材料（FGM），来制造牙科植入物。本研究旨在制造具有优异机械和生物学性能的 Ti-HA FGM 用于牙科植入物。
材料和方法
我们使用 HA 和 Ti 粉末制造了不同 Ti 体积分数（VF）的 Ti-HA FGM。通过研究其机械性能来表征 Ti-HA。使用细胞计数试剂盒-8 测定法和 LDH 细胞细胞毒性测定法来检测细胞毒性。在 XL30 环境扫描电子显微镜（ESEM）上进行扫描电子显微镜（SEM）观察。使用酶联免疫吸附测定（ELISA）试剂盒定量监测碱性磷酸酶（ALP）和转化生长因子（TGF-1）的表达。使用实时聚合酶链反应（PCR）测量 ALP 基因和 - 受体的表达。将 Ti-HA FGM 牙种植体植入比格犬。进行微计算机断层扫描（CT）和硬组织切片，以评估骨-植入物接触（BIC）和骨体积/总体积（BV/TV）。
结果
Ti-HA 的密度和机械性能呈现出与 VF 相对应的各种梯度分布。根据细胞计数试剂盒-8（CCK-8）和乳酸脱氢酶（LDH）测定的结果，两组之间的细胞毒性差异无统计学意义（=0.11）。ALP 和 TGF-1 水平略有上调。在第 7 天，Ti-HA 组的 ALP 和 TGF-RI 的转录水平高于 Ti 组，而 TGF-RII 的转录水平没有明显增加。Ti 和 Ti-HA FGM 组之间的 BIC 没有显着差异（=0.0504）。BV/TV 显示 Ti-HA FGM 组具有更好的成骨作用（=0.04）。
结论
Ti-HA FGM 有助于体内和体外牙科植入物的成骨作用。
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