Department of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, 4031 Debrecen, Hungary.
Department of Public Health and Epidemiology, Faculty of Medicine, University of Debrecen, 4028 Debrecen, Hungary.
Medicina (Kaunas). 2022 Jul 12;58(7):925. doi: 10.3390/medicina58070925.
: Endometriosis is a benign, chronic disease, that negatively influences the quality of life of affected women and is responsible for a remarkable amount of infertility. The pathophysiology of the disease is still not clarified, but the insufficient immune surveillance plays a significant role in it. The phagocyte function of innate immune cells may play a role in the elimination of ectopic endometrium. The purpose of this study is to examine the phagocyte function of neutrophil granulocytes and monocytes, incubated in heat-inactivated and not-inactivated plasma samples from healthy women and from women with endometriosis before and after the surgical treatment. : Blood samples were collected from eight preoperative and eight postoperative patients with endometriosis before and after the surgical treatment, and from 16 healthy patients as controls. Neutrophil granulocytes, monocytes and blood plasma samples were isolated. Cells were incubated in different plasma samples, and the phagocytic index was determined with a fluorescence microscope. The phagocytic index of granulocytes and monocytes isolated from patients with endometriosis was significantly decreased compared to healthy women after the cells were incubated in their own plasma. Preoperatively isolated cells from patients with endometriosis demonstrated an improved phagocyte function after incubating them in plasma samples from healthy controls. In contrast, the phagocytic activity of cells from healthy women significantly reduced after being incubated in the plasma of preoperative endometriosis patients. The heat-inactivation of plasma samples did not affect the results. Active endometriosis lesions may produce heat-stable systemic immunomodulatory factors, which reduced the phagocyte function of peripheral monocytes and neutrophil granulocytes. The phagocyte function of these cells can be normalized after the complete surgical removal of endometriosis, which then demonstrates similar values as in healthy women.
子宫内膜异位症是一种良性的慢性疾病，它会降低受影响女性的生活质量，并导致相当多的不孕。这种疾病的病理生理学仍不清楚，但免疫监视不足在其中起着重要作用。先天免疫细胞的吞噬细胞功能可能在异位子宫内膜的消除中发挥作用。本研究旨在检查在热失活和未失活的健康女性和子宫内膜异位症女性的血浆样本中孵育的中性粒细胞和单核细胞的吞噬细胞功能，以及在手术治疗前后。从八名术前和八名术后子宫内膜异位症患者以及 16 名健康患者中采集血液样本作为对照。分离中性粒细胞、单核细胞和血浆样本。将细胞在不同的血浆样本中孵育，并在荧光显微镜下确定吞噬指数。与健康女性相比，子宫内膜异位症患者的中性粒细胞和单核细胞的吞噬指数在自身血浆孵育后显著降低。与术前子宫内膜异位症患者的血浆相比，术前分离的子宫内膜异位症患者的细胞在健康对照组的血浆样本中孵育后，吞噬细胞功能得到改善。相比之下，健康女性细胞的吞噬活性在孵育术前子宫内膜异位症患者的血浆后显著降低。血浆样本的热失活并不影响结果。活动期子宫内膜异位症病变可能产生热稳定的全身免疫调节因子，降低外周单核细胞和中性粒细胞的吞噬细胞功能。这些细胞的吞噬细胞功能可以在子宫内膜异位症的完全手术切除后得到正常化，然后表现出与健康女性相似的值。
