Yang Da Hyun, Jung Sangyong, Kim Jae Young, Lee Nae Yoon
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Korea.
Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore.
Micromachines (Basel). 2022 Jul 15;13(7):1122. doi: 10.3390/mi13071122.
In this study, we fabricated a poly(dimethylsiloxane) (PDMS) surface coated with polydopamine (PDA) to enhance cell adhesion. PDA is well known for improving surface adhesion on various surfaces due to the abundant reactions enabled by the phenyl, amine, and catechol groups contained within it. To confirm the successful surface coating with PDA, the water contact angle and X-ray photoelectron spectroscopy were analyzed. Human umbilical vein endothelial cells (HUVECs) and human-bone-marrow-derived mesenchymal stem cells (MSCs) were cultured on the PDA-coated PDMS surface to evaluate potential improvements in cell adhesion and proliferation. HUVECs were also cultured inside a cylindrical PDMS microchannel, which was constructed to mimic a human blood vessel, and their growth and performance were compared to those of cells grown inside a rectangular microchannel. This study provides a helpful perspective for building a platform that mimics in vivo environments in a more realistic manner.
在本研究中，我们制备了一种涂有聚多巴胺（PDA）的聚二甲基硅氧烷（PDMS）表面，以增强细胞黏附。PDA因其所含的苯基、胺基和邻苯二酚基团能够引发丰富的反应而在改善各种表面的黏附性方面广为人知。为了确认PDA成功包覆于表面，我们分析了水接触角和X射线光电子能谱。将人脐静脉内皮细胞（HUVECs）和人骨髓间充质干细胞（MSCs）培养在涂有PDA的PDMS表面，以评估细胞黏附和增殖方面的潜在改善。HUVECs还被培养在一个圆柱形的PDMS微通道内，该微通道被构建用于模拟人体血管，并将其生长和性能与在矩形微通道内生长的细胞进行比较。本研究为构建一个更逼真地模拟体内环境的平台提供了有益的视角。
