Ozbolat Veli, Dey Madhuri, Ayan Bugra, Povilianskas Adomas, Demirel Melik C, Ozbolat Ibrahim T
Mechanical Engineering Department, Ceyhan Engineering Faculty, Cukurova University, Adana 01950, Turkey.
ACS Biomater Sci Eng. 2018 Feb 12;4(2):682-693. doi: 10.1021/acsbiomaterials.7b00646. Epub 2018 Jan 8.
Despite extensive use of polydimethylsiloxane (PDMS) in medical applications, such as lab-on-a-chip or tissue/organ-on-a-chip devices, point-of-care devices, and biological machines, the manufacturing of PDMS devices is limited to soft-lithography and its derivatives, which prohibits the fabrication of geometrically complex shapes. With the recent advances in three-dimensional (3D) printing, use of PDMS for fabrication of such complex shapes has gained considerable interest. This research presents a detailed investigation on printability of PDMS elastomers over three concentrations for mechanical and cell adhesion studies. The results demonstrate that 3D printing of PDMS improved the mechanical properties of fabricated samples up to three fold compared to that of cast ones because of the decreased porosity of bubble entrapment. Most importantly, 3D printing facilitates the adhesion of breast cancer cells, whereas cast samples do not allow cellular adhesion without the use of additional coatings such as extracellular matrix proteins. Cells are able to adhere and grow in the grooves along the printed filaments demonstrating that 3D printed devices can be engineered with superior cell adhesion qualities compared to traditionally manufactured PDMS devices.
尽管聚二甲基硅氧烷（PDMS）在医学应用中得到了广泛使用，如芯片实验室或组织/器官芯片设备、即时检测设备和生物机器，但PDMS设备的制造仅限于软光刻及其衍生技术，这限制了几何形状复杂的器件的制造。随着三维（3D）打印技术的最新进展，使用PDMS制造此类复杂形状引起了人们的极大兴趣。本研究对三种浓度的PDMS弹性体在机械性能和细胞粘附研究方面的可打印性进行了详细调查。结果表明，由于气泡截留孔隙率的降低，与浇铸样品相比，PDMS的3D打印使制造样品的机械性能提高了两倍。最重要的是，3D打印促进了乳腺癌细胞的粘附，而浇铸样品在不使用细胞外基质蛋白等额外涂层的情况下不允许细胞粘附。细胞能够沿着打印细丝在凹槽中粘附和生长，这表明与传统制造的PDMS设备相比，3D打印设备可以设计出具有卓越细胞粘附质量的产品。
