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来自GG的孢子展示p75蛋白对HT-29细胞转录反应的影响。

Effects of Spore-Displayed p75 Protein from GG on the Transcriptional Response of HT-29 Cells.

作者信息

Kang Soo-Ji, Kim Min-Joo, Son Do-Yeong, Kang Seok-Seong, Hong Kwang-Won

机构信息

Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Goyang-si 10326, Korea.

出版信息

Microorganisms. 2022 Jun 23;10(7):1276. doi: 10.3390/microorganisms10071276.

DOI:10.3390/microorganisms10071276
PMID:35888995
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9323162/
Abstract

A GG-derived protein, p75, is one of the key molecules exhibiting probiotic activity. However, the molecular mechanism and transcriptional response of p75 in human intestinal epithelial cells are not completely understood. To gain a deeper understanding of its potential probiotic action, this study investigated genome-wide responses of HT-29 cells to stimulation by spore-displayed p75 (CotG-p75) through a transcriptome analysis based on RNA sequencing. Analysis of RNA-seq data showed significant changes of gene expression in HT-29 cells stimulated by CotG-p75 compared to the control. A total of 189 up-regulated and 314 down-regulated genes was found as differentially expressed genes. Gene ontology enrichment analysis revealed that a large number of activated genes was involved in biological processes, such as epithelial cell differentiation, development, and regulation of cell proliferation. A gene-gene interaction network analysis showed that several DEGs, including , , , , , , , , , , , , , , , , , and , might play a critical role in these biological processes. RNA-seq results for selected genes were verified by reverse transcription-quantitative polymerase chain reaction. Overall, these results provide extensive knowledge about the transcriptional responses of HT-29 cells to stimulation by CotG-p75. This study showed that CotG-p75 can contribute to cell survival and epithelial development in human intestinal epithelial cells.

摘要

一种源自嗜热栖热放线菌（GG）的蛋白质p75是表现出益生菌活性的关键分子之一。然而，p75在人肠道上皮细胞中的分子机制和转录反应尚未完全明确。为了更深入了解其潜在的益生菌作用，本研究通过基于RNA测序的转录组分析，研究了HT-29细胞对孢子展示的p75（CotG-p75）刺激的全基因组反应。RNA测序数据分析显示，与对照相比，CotG-p75刺激的HT-29细胞中基因表达有显著变化。共发现189个上调基因和314个下调基因作为差异表达基因。基因本体富集分析表明，大量激活基因参与上皮细胞分化、发育和细胞增殖调控等生物学过程。基因-基因相互作用网络分析表明，包括……等在内的几个差异表达基因可能在这些生物学过程中起关键作用。通过逆转录-定量聚合酶链反应对选定基因的RNA测序结果进行了验证。总体而言，这些结果提供了关于HT-29细胞对CotG-p75刺激的转录反应的广泛知识。本研究表明，CotG-p75有助于人肠道上皮细胞的细胞存活和上皮发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/b1a03b56f5fd/microorganisms-10-01276-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/626878340493/microorganisms-10-01276-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/3b4982bb11d2/microorganisms-10-01276-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/d3c06d91c34a/microorganisms-10-01276-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/90e25f8bc8f7/microorganisms-10-01276-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/d320d9da9b45/microorganisms-10-01276-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/c6d63d59ba80/microorganisms-10-01276-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/72372fbdc914/microorganisms-10-01276-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/d21d5e843a57/microorganisms-10-01276-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/b1a03b56f5fd/microorganisms-10-01276-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/626878340493/microorganisms-10-01276-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/3b4982bb11d2/microorganisms-10-01276-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/d3c06d91c34a/microorganisms-10-01276-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/90e25f8bc8f7/microorganisms-10-01276-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/d320d9da9b45/microorganisms-10-01276-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/c6d63d59ba80/microorganisms-10-01276-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/72372fbdc914/microorganisms-10-01276-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/d21d5e843a57/microorganisms-10-01276-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e19/9323162/b1a03b56f5fd/microorganisms-10-01276-g009.jpg

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