Guoyan Zhang, Yingfeng An, Zabed Hossain, Qi Guo, Yang Miaomiao, Jiao Yuan, Li Wen, Wenjing Sun, Xianghui Qi
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, P.R. China.
College of Biosciences and Biotechnology, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110161, Liaoning, P.R. China.
J Microbiol Biotechnol. 2019 Feb 28;29(2):179-190. doi: 10.4014/jmb.1807.06066.
spore surface display (BSSD) technology is considered to be one of the most promising approaches for expressing heterologous proteins with high activity and stability. Currently, this technology is used for various purposes, such as the production of enzymes, oral vaccines, drugs and multimeric proteins, and the control of environmental pollution. This paper presents an overview of the latest developments in BSSD technology and its application in protein engineering. Finally, the major limitations of this technology and future directions for its research are discussed.
孢子表面展示（BSSD）技术被认为是表达具有高活性和稳定性的异源蛋白最具前景的方法之一。目前，该技术用于多种目的，如酶、口服疫苗、药物和多聚体蛋白的生产以及环境污染控制。本文概述了BSSD技术的最新进展及其在蛋白质工程中的应用。最后，讨论了该技术的主要局限性及其未来的研究方向。
