Nees Marina, Hess Michael, Hess Claudia
Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
Microorganisms. 2022 Jul 20;10(7):1472. doi: 10.3390/microorganisms10071472.
, a major pathogen in poultry production, is involved in femoral head necrosis (FHN) in broiler birds. So far, the characterization and relationship of isolates in context with this disease are mainly based on phenotypic and genotypic characteristics. Previously, an involvement of diverse isolates was reported. MALDI-TOF MS has been successfully applied investigating the clonality of different bacteria. Therefore, its application to characterize a well-defined selection of isolates beyond the species level was tested. The isolates were derived from clinical cases of FHN as well as from healthy birds. Reproducibility studies to perform a standardized protocol were done, and LB agar as well as the usage of fresh bacterial cultures proved most appropriate. No distinct clustering in context with the origin of isolates, association with lesions, serotype, or PFGE profile was found. Most of the isolates belonging to phylogroup B2 revealed a characteristic peak shift at 9716 / and could be attributed to the same MALDI-TOF MS cluster. The present study confirmed the previously found pheno- and genotypic heterogeneity of involved in FHN on the proteomic level. The study also highlights the need for standardized protocols when using MALDI-TOF MS for bacterial typing, especially beyond species level.
作为家禽生产中的主要病原体，与肉鸡股骨头坏死（FHN）有关。到目前为止，与该疾病相关的分离株的特征及关系主要基于表型和基因型特征。此前，有报道称多种分离株与之有关。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已成功应用于研究不同细菌的克隆性。因此，测试了其在物种水平之外对明确选择的分离株进行特征描述的应用。这些分离株来源于FHN临床病例以及健康鸟类。进行了重现性研究以执行标准化方案，结果表明LB琼脂以及使用新鲜细菌培养物最为合适。未发现与分离株来源、与病变的关联、血清型或脉冲场凝胶电泳（PFGE）图谱相关的明显聚类。大多数属于B2系统发育群的分离株在9716 /处显示出特征性的峰移，并且可归因于同一个MALDI-TOF MS聚类。本研究在蛋白质组水平上证实了先前发现的与FHN相关的表型和基因型异质性。该研究还强调了在使用MALDI-TOF MS进行细菌分型时，尤其是在物种水平之外，需要标准化方案。
