膳食植物化学大豆皂甙 I 的抗结肠癌活性及其对 HCT116 代谢重编程的诱导作用。
Anti-Colon Cancer Activity of Dietary Phytochemical Soyasaponin I and the Induction of Metabolic Shifts in HCT116.
机构信息
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
出版信息
Molecules. 2022 Jul 8;27(14):4382. doi: 10.3390/molecules27144382.
Dietary phytochemicals play an important role in the prevention and treatment of colon cancer. It is reported that group B of soyasaponin, derived from dietary pulses, has anti-colonic effects on some colon cancer cell lines. However, it is uncertain which specific soybean saponins play a role. In our study, as one of the group B soyasaponin, the anti-colon cancer activity of soyasaponins I (SsI) was screened, and we found that it had the inhibitory effect of proliferation on colon cancer cell lines HCT116 (IC = 161.4 μM) and LoVo (IC = 180.5 μM), but no effect on HT29 between 0-200 μM. Then, nine potential targets of SsI on colon cancer were obtained by network pharmacology analysis. A total of 45 differential metabolites were identified by metabolomics analysis, and the KEGG pathway was mainly enriched in the pathways related to the absorption and metabolism of amino acids. Finally, molecular docking analysis predicted that SsI might dock with the protein of DNMT1, ERK1. The results indicated that the effect of SsI on HCT116 might be exerted by influencing amino acid metabolism and the estrogen signaling pathway. This study may provide the possibility for the application of SsI against colon cancer.
膳食植物化学物质在结肠癌的预防和治疗中发挥着重要作用。据报道，来源于食用豆类的大豆皂素 B 组对一些结肠癌细胞系具有抗结肠作用。然而，目前还不确定哪种特定的大豆皂素有作用。在我们的研究中，作为 B 组大豆皂素之一，筛选了大豆皂素 I（SsI）的抗结肠癌活性，我们发现它对结肠癌细胞系 HCT116（IC=161.4 μM）和 LoVo（IC=180.5 μM）的增殖具有抑制作用，但在 0-200 μM 范围内对 HT29 没有影响。然后，通过网络药理学分析获得了 SsI 对结肠癌的 9 个潜在靶点。通过代谢组学分析共鉴定出 45 个差异代谢物，KEGG 途径主要富集在与氨基酸吸收和代谢相关的途径中。最后，分子对接分析预测 SsI 可能与 DNMT1、ERK1 的蛋白结合。结果表明，SsI 对 HCT116 的作用可能是通过影响氨基酸代谢和雌激素信号通路来发挥的。本研究可能为 SsI 防治结肠癌提供了可能性。
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