Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
Key Laboratory of Environmental Medicine and Engineering (Ministry of Education), School of Public Health, Southeast University, Nanjing 210096, China.
Molecules. 2022 Jul 10;27(14):4417. doi: 10.3390/molecules27144417.
The 8-iso-prostaglandin F2α (8-iso-PGF2α) biomarker is used as the gold standard for tracing lipid oxidative stress in vivo. The analysis of urinary 8-iso-PGF2α is challenging when dealing with trace amounts of 8-iso-PGF2α and the complexity of urine matrixes. A packed-fiber solid-phase extraction (PFSPE)−coupled with HPLC-MS/MS−method, based on polystyrene (PS)-electrospun nanofibers, was developed for the specific determination of 8-iso-PGF2α in urine and compared with other newly developed LC-MS/MS methods. The method, which simultaneously processed 12 samples within 5 min on a self-made semi-automatic array solid-phase extraction processor, was the first to introduce PS-electrospun nanofibers as an adsorbent for the extraction of 8-iso-PGF2α and was successfully applied to real urine samples. After optimizing the PFSPE conditions, good linearity in the range of 0.05−5 ng/mL with R2 > 0.9996 and a satisfactory limit of detection of 0.015 ng/mL were obtained, with good intraday and interday precision (RSD < 10%) and recoveries of 95.3−103.8%. This feasible method is expected to be used for the batch quantitative analysis of urinary 8-iso-PGF2α.
8-异前列腺素 F2α（8-iso-PGF2α）生物标志物被用作体内脂质氧化应激追踪的金标准。在痕量 8-iso-PGF2α 和尿液基质复杂性的情况下，分析尿液中的 8-iso-PGF2α 具有挑战性。本研究开发了一种基于聚苯乙烯（PS）电纺纳米纤维的填充纤维固相萃取（PFSPE）-高效液相色谱-串联质谱（HPLC-MS/MS）方法，用于特异性测定尿液中的 8-iso-PGF2α，并与其他新开发的 LC-MS/MS 方法进行了比较。该方法在自制半自动阵列固相萃取处理器上 5 分钟内同时处理 12 个样品，首次将 PS 电纺纳米纤维用作提取 8-iso-PGF2α 的吸附剂，并成功应用于实际尿液样品。在优化 PFSPE 条件后，获得了在 0.05-5 ng/mL 范围内具有良好线性（R2 > 0.9996）和令人满意的检测限（0.015 ng/mL），日内和日间精密度（RSD < 10%）良好，回收率为 95.3-103.8%。该可行的方法有望用于批量定量分析尿液中的 8-iso-PGF2α。
