Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea.
Molecules. 2022 Jul 20;27(14):4636. doi: 10.3390/molecules27144636.
In contrast to the stem and fruit of , leaves as a source rich in phenolic compounds with potentially beneficial pharmacological activities have been largely overlooked. To develop and use leaves as a resource, we evaluated its potential as a cardiovascular-protective agent. Herein, we investigated the effects and potential mechanisms of leaves extract on lipopolysaccharide (LPS)-induced inflammatory responses in human umbilical vein endothelial cells. We found that leaves extract pretreatment of 10 μg/mL significantly attenuated LPS-induced protein expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1. Furthermore, this extract also suppressed LPS-induced phosphorylation of nuclear factor-κB p65. In order to elucidate the chemical profiles of the samples, the HPLC fingerprint was established, and prominent peaks were identified via HPLC-electrospray ionization-mass spectrometry. Multivariate statistical analyses, including hierarchical cluster analysis, principal component analysis, and partial least-squares discriminant analysis, were performed to evaluate the clustering of the samples. It was found that isochlorogenic acid C was a key marker for the classification of leaves from the Gongju and Muju city in Korea. Collectively, this study not only suggested the potential of leaves as a novel therapeutic candidate for inflammatory cardiovascular disease but also developed a quality control method for leaves, which could help to expand the application of .
与 和果实相比， 叶子作为一种富含酚类化合物的来源，具有潜在的有益的药理活性，但在很大程度上被忽视了。为了开发和利用 叶子作为一种资源，我们评估了它作为一种心血管保护剂的潜力。在此，我们研究了 叶子提取物对脂多糖 (LPS) 诱导的人脐静脉内皮细胞炎症反应的影响及其潜在机制。我们发现， 叶子提取物预处理 10 μg/mL 可显著减弱 LPS 诱导的细胞间黏附分子-1 和血管细胞黏附分子-1 的蛋白表达。此外，这种提取物还抑制了 LPS 诱导的核因子-κB p65 的磷酸化。为了阐明样品的化学特征，建立了 HPLC 指纹图谱，并通过 HPLC-电喷雾电离质谱鉴定了突出的峰。采用层次聚类分析、主成分分析和偏最小二乘判别分析等多元统计分析方法对样品进行了聚类评估。结果表明，异绿原酸 C 是韩国公州和木莒市 叶子分类的关键标志物。总之，这项研究不仅表明 叶子作为一种治疗炎症性心血管疾病的新型候选药物具有潜力，而且还开发了一种 叶子的质量控制方法，这有助于扩大 的应用。
