College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China.
College of Modern Science and Technology, China Jiliang University, Hangzhou, Zhejiang 310018, China.
Int J Biol Macromol. 2021 Jan 15;167:423-433. doi: 10.1016/j.ijbiomac.2020.11.160. Epub 2020 Nov 27.
The present study was designed to explore the in vivo-antioxidant capacity and the probable mechanism of AAPs-H, prepared from Auricularia auricula polysaccharides with the optimal extraction conditions by Box-Behnken design and acid hydrolysis, using Caenorhabditis elegans as a model organism. The effects of AAPs-H on the locomotion behavior, life span, antioxidant-related enzymes activities, and antioxidants levels in C. elegans were studied. Furthermore, the potentials of AAPs-H in up-regulating the expression of antioxidant-related genes in C. elegans, such as skn-1, sod-3 and sir-2.1, were also discussed. AAPs-H demonstrated a highly significant protective effect against the damage caused by paraquat, could significantly increase U-Turn frequency of worms (p < 0.01), extend their lifespan, enhance antioxidant systems including GR by 63.96% (p < 0.05), GSH-Px by 71.16% (p < 0.01), SOD by 78.65% (p < 0.01) and CAT by 98.52% (p < 0.01), increase the level of GSH by 28.12% (p < 0.05), and decrease the level of MDA by 39.29% (p < 0.01). The qRT-PCR results showed that AAPs-H could up regulate mRNA expression levels of skn-1, sod-1, sod-2, sod-3 and sir-2.1 in wild-type C. elegans (>1.6 fold) when treated with the concentration of 0.4 mg/mL (p < 0.05 or p < 0.01). Our studies provide evidence that AAPs-H improves antioxidant defense system, and up-regulation of oxidative stress related genes for prevention of stress damage in C. elegans.
本研究旨在探索从最佳提取条件的银耳多糖制备的 AAPs-H 的体内抗氧化能力及其可能的机制，采用秀丽隐杆线虫作为模型生物。研究了 AAPs-H 对线虫的运动行为、寿命、抗氧化相关酶活性和抗氧化剂水平的影响。此外，还探讨了 AAPs-H 在线虫体内上调抗氧化相关基因 skn-1、sod-3 和 sir-2.1 表达的潜力。AAPs-H 对百草枯引起的损伤表现出高度显著的保护作用，能显著增加线虫的 U 型转弯频率（p<0.01），延长其寿命，增强抗氧化系统，包括 GR 增加 63.96%（p<0.05）、GSH-Px 增加 71.16%（p<0.01）、SOD 增加 78.65%（p<0.01）和 CAT 增加 98.52%（p<0.01），GSH 水平增加 28.12%（p<0.05），MDA 水平降低 39.29%（p<0.01）。qRT-PCR 结果表明，AAPs-H 可在浓度为 0.4mg/mL 时上调野生型秀丽隐杆线虫中 skn-1、sod-1、sod-2、sod-3 和 sir-2.1 的 mRNA 表达水平（>1.6 倍）（p<0.05 或 p<0.01）。我们的研究结果表明，AAPs-H 可改善抗氧化防御系统，并上调氧化应激相关基因，以防止秀丽隐杆线虫的应激损伤。
