Sayed Rafik Hamed, Abousenna Mohamed Samy, Elsaady Shaimaa Abdelall, Soliman Rafik, Saad Mohamed Ahmed
Central Laboratory for Evaluation of Veterinary Biologics, Agricultural Research Center, P.O. Box 131, Cairo 11381, Egypt.
Department of Microbiology, Faculty of Veterinary medicine, Cairo University, Giza 12211, Egypt.
Nanomaterials (Basel). 2022 Jul 19;12(14):2477. doi: 10.3390/nano12142477.
In the presented study, we developed a nanogold lateral glow immunoassay-based technique (LFI-COVID-19 antigen test) for the detection of SARS-CoV-2 nucleocapsid proteins; the developed LFI-COVID-19 Ag test has been tested for limit of detection (LOD), cross-reactivity and interfering substances, and performance. It was found that the performance of the developed LFI-COVID-19 antigen test when it was evaluated by RT-qPCR indicated 95, 98, and 97% for sensitivity, specificity and accuracy, respectively. This complies with the WHO guidelines. It was concluded that the developed LFI-COVID-19 antigen test is a point of care and an alternative approach to current laboratory methods, especially RT-qPCR. It provides an easy, rapid (within 20 min), and on-site diagnostic tool for COVID-19 infection, and it is a cheap test if it is manufactured on a large scale for commercial use.
在本研究中，我们开发了一种基于纳米金侧向免疫发光分析技术（LFI-COVID-19抗原检测）来检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核衣壳蛋白；所开发的LFI-COVID-19抗原检测已针对检测限（LOD）、交叉反应性、干扰物质和性能进行了测试。结果发现，通过逆转录定量聚合酶链反应（RT-qPCR）评估时，所开发的LFI-COVID-19抗原检测的灵敏度、特异性和准确性分别为95%、98%和97%。这符合世界卫生组织的指南。结论是，所开发的LFI-COVID-19抗原检测是一种即时检测方法，是当前实验室方法（尤其是RT-qPCR）的替代方法。它为新型冠状病毒肺炎（COVID-19）感染提供了一种简便、快速（20分钟内）的现场诊断工具，如果大规模生产用于商业用途，它是一种廉价的检测方法。
