Central Laboratory for Evaluation of Veterinary Biologics, Agricultural Research Center, P.O. Box 131, Cairo, 11381, Egypt.
Sci Rep. 2024 Mar 1;14(1):5060. doi: 10.1038/s41598-024-55548-x.
In this study, we devised a nanogold lateral flow immunoassay (LFA-CPV antigen test) for detecting canine parvovirus (CPV) in living attenuated CPV vaccines. We conducted instrumental characterization of the prepared nanogold particles and the developed LFA-CPV antigen test was rigorously evaluated for its performance verification including limit of detection, sensitivity, specificity, selectivity and accuracy. The LFA-CPV antigen test demonstrated strong performance when assessed against qPCR using different batches of live attenuated CPV vaccines, indicated a sensitivity of 96.4%, specificity of 88.2%, and an overall accuracy of 95%. These results suggest that the developed LFA-CPV antigen test could serve as a viable alternative for evaluation live attenuated CPV vaccines, and provide it as a point of care test for CPV diagnosis, offering a potential substitute for traditional laboratory methods, particularly qPCR.
在这项研究中，我们设计了一种纳米金侧向流动免疫分析（LFA-CPV 抗原检测），用于检测活弱毒 CPV 疫苗中的犬细小病毒（CPV）。我们对制备的纳米金颗粒进行了仪器表征，并对开发的 LFA-CPV 抗原检测进行了严格评估，以验证其性能，包括检测限、灵敏度、特异性、选择性和准确性。该 LFA-CPV 抗原检测在使用不同批次活弱毒 CPV 疫苗进行 qPCR 评估时表现出较强的性能，表明其灵敏度为 96.4%，特异性为 88.2%，总准确率为 95%。这些结果表明，开发的 LFA-CPV 抗原检测可作为活弱毒 CPV 疫苗评估的一种可行替代方法，并将其作为 CPV 诊断的即时护理检测方法，为传统实验室方法，特别是 qPCR 提供了潜在替代品。
