Torrejón Estefania, Sanches Gustavo Seron, Moerbeck Leonardo, Santos Lenira, André Marcos Rogério, Domingos Ana, Antunes Sandra
Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal.
Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição 1155, Curitiba 80215-901, PR, Brazil.
Pathogens. 2022 Jun 30;11(7):749. doi: 10.3390/pathogens11070749.
spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for spp. has not been totally understood. Considering only a few studies had a focus on screening in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the gene, it was possible to detect sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as , 11 as and 1 presented co-infection with both species. The absolute quantification of DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 10 copies/µL. The sampled dogs, humans and ticks were negative. These results showed that and are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats.
[该菌属]包含新出现和再次出现的苛养革兰氏阴性菌，分布于全球。猫是[该菌属细菌]的主要储存宿主，而狗是该细菌的机会性宿主。尽管蜱虫可能也在传播中起作用，但它们作为[该菌属细菌]传播媒介的能力尚未完全明确。考虑到葡萄牙仅有少数研究关注动物、人类和体外寄生虫中[该菌属细菌]的筛查，本研究旨在确定[该菌属细菌]在葡萄牙123只流浪猫、25只流浪狗、30名来自里斯本的人和236只采获蜱中的分子存在情况。使用针对[某基因]的qPCR方法，在20.32%的猫样本（25/123）中检测到了[该菌属细菌]的DNA。在这些阳性样本中，13个序列被鉴定为[某种菌]，11个为[另一种菌]，1个呈现两种菌的共感染。采样猫中[该菌属细菌]DNA的绝对定量范围为2.78×10至1.03×10拷贝/微升。采样的狗、人和蜱均为阴性。这些结果表明[某种菌]和[另一种菌]在里斯本的流浪猫中传播。应开展更多更广泛的研究，以确定此类感染对人类，特别是那些与猫持续直接接触的人的影响。
