Abd El-Fattah Wesam, Alfaifi Mohammad Y, Alkabli Jafar, Ramadan Heba A, Shati Ali A, Elbehairi Serag Eldin I, Elshaarawy Reda F M, Kamal Islam, Saleh Moustafa M
Chemistry Department, College of Science, IMSIU (Imam Mohammad Ibn Saud Islamic University), P.O. Box 5701, Riyadh 11432, Saudi Arabia.
Department of Chemistry, Faculty of Science, Port Said University, Port Said 42521, Egypt.
Antibiotics (Basel). 2023 Jun 27;12(7):1110. doi: 10.3390/antibiotics12071110.
This study presents a green protocol for the fabrication of a multifunctional smart nanobiocomposite (NBC) (ZnO-PIACSB-TiO) for secure antimicrobial and antibiofilm applications. First, shrimp shells were upgraded to a polyimidazolium amphiphilic chitosan Schiff base (PIACSB) through a series of physicochemical processes. After that, the PIACSB was used as an encapsulating and coating agent to manufacture a hybrid NBC in situ by co-encapsulating ZnONPs and TiONPs. The physicochemical and visual characteristics of the new NBC were investigated by spectral, microscopic, electrical, and thermal methods. The antimicrobial indices revealed that the newly synthesized, PIACSB-coated TiO-ZnO nanocomposite is an exciting antibiotic due to its amazing antimicrobial activity (MIC/MBC→0.34/0.68 μg/mL, 0.20/0.40 μg/mL, and 0.15/0.30 μg/mL working against , , and , respectively) and antifungal capabilities. Additionally, ZnO-PIACSB-TiO2 is a potential fighter of bacterial biofilms, with the results being superior to those of the positive control (Cipro), which worked against (only 8.7% ± 1.9 biofilm growth), (only 1.4% ± 1.1 biofilm growth), and (only 0.85% ± 1.3 biofilm growth). Meanwhile, the NBC exhibits excellent biocompatibility, as evidenced by its IC values against both L929 and HSF (135 and 143 µg/mL), which are significantly higher than those of the MIC doses (0.24-24.85 µg/mL) that work against all tested microbes, as well as the uncoated nanocomposite (IC = 19.36 ± 2.04 and 23.48 ± 1.56 µg/mL). These findings imply that the new PIACSB-coated nanocomposite film may offer promising multifunctional food packaging additives to address the customer demand for safe, eco-friendly food products with outstanding antimicrobial and antibiofilm capabilities.
本研究提出了一种用于制备多功能智能纳米生物复合材料（NBC）（ZnO-PIACSB-TiO）的绿色方案，用于安全的抗菌和抗生物膜应用。首先，通过一系列物理化学过程将虾壳升级为聚咪唑鎓两亲性壳聚糖席夫碱（PIACSB）。之后，PIACSB用作封装和涂层剂，通过共封装ZnONPs和TiONPs原位制造混合NBC。通过光谱、显微镜、电学和热学方法研究了新型NBC的物理化学和视觉特性。抗菌指标显示，新合成的PIACSB涂层TiO-ZnO纳米复合材料是一种令人兴奋的抗生素，因为它具有惊人的抗菌活性（MIC/MBC分别为→0.34/0.68μg/mL、0.20/0.40μg/mL和0.15/0.30μg/mL，分别对、和有效）和抗真菌能力。此外，ZnO-PIACSB-TiO2是细菌生物膜的潜在克星，其结果优于阳性对照（环丙沙星），对（仅8.7%±1.9生物膜生长）、（仅1.4%±1.1生物膜生长）和（仅0.85%±1.3生物膜生长）有效。同时，NBC表现出优异的生物相容性，其对L929和HSF的IC值（135和143μg/mL）证明了这一点，这显著高于对所有测试微生物有效的MIC剂量（0.24-24.85μg/mL）以及未涂层的纳米复合材料（IC = 19.36±2.04和23.48±1.56μg/mL）。这些发现表明，新的PIACSB涂层纳米复合膜可能提供有前景的多功能食品包装添加剂，以满足客户对具有出色抗菌和抗生物膜能力的安全、环保食品的需求。
