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富含类黄酮的叶片提取物可抑制生物膜形成和菌丝生长。

Flavonoid-Rich Fractions of Leaves Inhibit Biofilm Formation and Hyphae Growth.

作者信息

da Fonseca Sara Thamires Dias, Teixeira Thaiz Rodrigues, Ferreira Jaqueline Maria Siqueira, Lima Luciana Alves Rodrigues Dos Santos, Luyten Walter, Castro Ana Hortência Fonsêca

机构信息

Laboratory of Natural Products, Postgraduate Program in Biotechnology, Campus Centro-Oeste, Universidade Federal de São João del-Rei, Divinópolis 35501-296, MG, Brazil.

Department of Biomolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-900, SP, Brazil.

出版信息

Plants (Basel). 2022 Jul 7;11(14):1796. doi: 10.3390/plants11141796.

DOI:10.3390/plants11141796
PMID:35890430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9322443/
Abstract

This study evaluated the effect of the extract and fractions of on planktonic growth, biofilm formation, mature biofilm, and hyphae growth. Three strains (SC5314, ATCC 18804, and ATCC 10231) were tested. The crude extract and the fractions were obtained by exhaustive percolation and liquid-liquid partition, respectively. Phytochemical analyses of extract and fractions were performed using high-performance liquid chromatography coupled with a diode-array detector and mass spectrometry (HPLC-DAD-MS). A microdilution assay was used to evaluate the effect of the extract and fractions on planktonic growth, and crystal violet staining was used to measure the total biomass of the biofilm. Hyphae growth was analyzed using light microscopy. Thirteen flavonoids were identified, with a predominance of the flavonol-3--glycoside type based on quercetin, myricetin, and kaempferol. Flavonoid-rich fractions of leaves displayed antifungal activity and inhibited both biofilm formation and hyphae growth in all the tested strains, but were not effective on planktonic growth and mature biofilm. This study indicates that flavonoid-rich fractions from leaves interfere with the virulence of species and support the use of spp. in folk medicine to treat infections.

摘要

本研究评估了[植物名称]提取物及其馏分对[真菌名称]浮游生长、生物膜形成、成熟生物膜和菌丝生长的影响。测试了三株[真菌名称]菌株（SC5314、ATCC 18804和ATCC 10231）。粗提取物和馏分分别通过彻底渗滤和液-液分配获得。使用高效液相色谱结合二极管阵列检测器和质谱（HPLC-DAD-MS）对[植物名称]提取物及其馏分进行植物化学分析。采用微量稀释法评估[植物名称]提取物及其馏分对[真菌名称]浮游生长的影响，并用结晶紫染色法测量生物膜的总生物量。使用光学显微镜分析菌丝生长。鉴定出13种黄酮类化合物，主要为基于槲皮素、杨梅素和山奈酚的黄酮醇-3-糖苷类型。[植物名称]叶中富含黄酮类化合物的馏分具有抗真菌活性，可抑制所有测试菌株的生物膜形成和菌丝生长，但对[真菌名称]浮游生长和成熟生物膜无效。本研究表明，[植物名称]叶中富含黄酮类化合物的馏分可干扰[真菌名称]物种的毒力，并支持[植物名称]属在民间医学中用于治疗感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/8538d81232f1/plants-11-01796-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/4146e9d4a9c5/plants-11-01796-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/6258885353d9/plants-11-01796-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/f623f117d015/plants-11-01796-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/d949fc425e84/plants-11-01796-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/8538d81232f1/plants-11-01796-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/4146e9d4a9c5/plants-11-01796-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/6258885353d9/plants-11-01796-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/f623f117d015/plants-11-01796-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/d949fc425e84/plants-11-01796-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf32/9322443/8538d81232f1/plants-11-01796-g005.jpg

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