Department of Veterinary Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Chuo Kikuu, Morogoro P.O. Box 3019, Tanzania.
SACIDS Foundation for One Health, Sokoine University of Agriculture, Chuo Kikuu, Morogoro P.O. Box 3015, Tanzania.
Molecules. 2024 Oct 2;29(19):4684. doi: 10.3390/molecules29194684.
The plants , and are well known in traditional African medicine, and particularly in traditional Chadian medicine. They are commonly used to treat infectious diseases, inflammatory diseases, fevers, gastroenteritis and other medical conditions. The aim of this study was to perform a phytochemical screening to determine the antioxidant properties of different extracts and fractions from the three plants. Ethanolic extracts and solvent fractions were prepared and analyzed for total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC). LC-MS and an online screening HPLC-ABTS system identified phytochemicals with antioxidant activities. DPPH and ABTS reduction methods were used to test the extracts and fractions for their antioxidant potential. The results showed that the TPC of was higher than that of , ranging from 64.70 ± 5.2 to 411.16 ± 8.11 mgGAE/g DW. extracts and fractions, on the other hand, showed higher TFC, ranging from 69.5 ± 5.3 to 408.26 ± 8.42 mgQE/g DW, and higher TTC, ranging from 4.57 ± 2.45 to 62.19 ± 4.7 mgTAE/g DW. The maximum TPC, TFC and TTC in both plants were recorded in the ethyl acetate fractions. extracts and fractions showed a very low quantity of TPC, TFC and TTC. Based on LC-MS and HPLC-ABTS analysis, rosmarinic acid was identified as the major component in the extracts and all fractions of and epicatechin, procyanidin B and quercetin were found in . did not exhibit specific substances with antioxidant activity and was therefore not considered for further assays. DPPH and ABTS results showed that ethyl acetate fractions of and have the strongest antioxidant activities. This study indicates that and are good sources of phytochemicals with antioxidant properties, suitable for medicinal use in Chadian communities. Additionally, the antioxidant-rich extracts from these plants hold significant potential for cosmetic development, enhancing skin health and protecting against oxidative-stress-induced damage.
植物 和 在传统的非洲医学中广为人知，特别是在乍得传统医学中。它们通常用于治疗传染病、炎症性疾病、发热、肠胃炎和其他医疗状况。本研究的目的是进行植物化学筛选，以确定这三种植物的不同提取物和馏分的抗氧化特性。制备了乙醇提取物和溶剂馏分，并分析了总酚含量（TPC）、总黄酮含量（TFC）和总单宁含量（TTC）。LC-MS 和在线筛选 HPLC-ABTS 系统鉴定了具有抗氧化活性的植物化学物质。DPPH 和 ABTS 还原法用于测试提取物和馏分的抗氧化潜力。结果表明， 的 TPC 高于 ，范围为 64.70±5.2 至 411.16±8.11mgGAE/gDW。另一方面， 提取物和馏分显示出更高的 TFC，范围为 69.5±5.3 至 408.26±8.42mgQE/gDW，以及更高的 TTC，范围为 4.57±2.45 至 62.19±4.7mgTAE/gDW。这两种植物的 TPC、TFC 和 TTC 最大值均记录在乙酸乙酯馏分中。 和 的提取物和馏分仅显示出非常低量的 TPC、TFC 和 TTC。根据 LC-MS 和 HPLC-ABTS 分析，迷迭香酸被确定为提取物和所有馏分的主要成分，而表儿茶素、原花青素 B 和槲皮素则存在于 中。 没有表现出具有抗氧化活性的特定物质，因此不考虑进一步的测定。DPPH 和 ABTS 结果表明， 和 的乙酸乙酯馏分具有最强的抗氧化活性。本研究表明， 和 是具有抗氧化特性的植物化学物质的良好来源，适合乍得社区的药用。此外，这些植物中富含抗氧化剂的提取物具有显著的潜力可用于化妆品开发，增强皮肤健康并预防氧化应激引起的损伤。
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