Elbagory Mohssen, El-Nahrawy Sahar, Omara Alaa El-Dein
Department of Biology, Faculty of Science and Arts, King Khalid University, Mohail 61321, Assir, Saudi Arabia.
Agricultural Research Center, Department of Microbiology, Soils, Water and Environment Research Institute, Giza 12112, Egypt.
Plants (Basel). 2022 Jul 9;11(14):1812. doi: 10.3390/plants11141812.
Several activities in the agriculture sector lead to the accumulation of Nickel (Ni) in soil. Therefore, effective and economical ways to reduce soil bioavailability of Ni must be identified. Five isolates of Rhizobium leguminosarum biovar Viceae (ICARDA 441, ICARDA 36, ICARDA 39, TAL−1148, and ARC−207) and three bacterial strains (Bacillus subtilis, B. circulance, and B. coagulans) were evaluated for tolerance and biosorption of different levels of Ni (0, 20, 40, 60, and 80 mg L−1). Pot experiments were conducted during the 2019/2020 and 2020/2021 seasons using four inoculation treatments (inoculation with the most tolerant Rhizobium (TAL−1148), inoculation with the most tolerant Rhizobium (TAL−1148) + B. subtilis, inoculation with the most tolerant Rhizobium (TAL−1148) + B. circulance, and inoculation with the most tolerant Rhizobium (TAL−1148) + B. coagulans) under different levels of Ni (0, 200, 400, and 600 mg kg−1), and their effects on growth, physiological characteristics, antioxidant enzymes, and Ni accumulation in faba bean plants (Vicia faba C.V. Nobaria 1) were determined. The results showed that Rhizobium (TAL−1148) and B. subtilis were the most tolerant of Ni. In pot trials, inoculation with the most tolerant Rhizobium TAL−1148 + B. subtilis treatment was shown to be more effective in terms of growth parameters (dry weight of plant, plant height, number of nodules, and N2 content), and this was reflected in physiological characteristics and antioxidant enzymes under 600 mg kg−1 Ni compared to the other treatments in the 2019/2020 season. In the second season, 2020/2021, a similar pattern was observed. Additionally, lower concentrations of Ni were found in faba bean plants (roots and shoots). Therefore, a combination of the most tolerant Rhizobium (TAL−1148) + B. subtilis treatment might be used to reduce Ni toxicity.
农业部门的多项活动会导致土壤中镍（Ni）的积累。因此，必须找到有效且经济的方法来降低土壤中镍的生物有效性。对五株豌豆根瘤菌生物变种豌豆（ICARDA 441、ICARDA 36、ICARDA 39、TAL−1148和ARC−207）和三株细菌菌株（枯草芽孢杆菌、环状芽孢杆菌和凝结芽孢杆菌）进行了不同水平镍（0、20、40、60和80 mg L−1）耐受性和生物吸附的评估。在2019/2020年和2020/2021年季节进行了盆栽试验，采用四种接种处理（接种耐受性最强的根瘤菌（TAL−1148）、接种耐受性最强的根瘤菌（TAL−1148）+枯草芽孢杆菌、接种耐受性最强的根瘤菌（TAL−1148）+环状芽孢杆菌、接种耐受性最强的根瘤菌（TAL−1148）+凝结芽孢杆菌），设置不同水平的镍（0、200、400和600 mg kg−1），并测定它们对蚕豆植株（蚕豆品种Nobaria 1）生长、生理特性、抗氧化酶和镍积累的影响。结果表明，根瘤菌（TAL−1148）和枯草芽孢杆菌对镍的耐受性最强。在盆栽试验中，与2019/2020年季节的其他处理相比，接种耐受性最强的根瘤菌TAL−1148+枯草芽孢杆菌处理在生长参数（植株干重、株高、根瘤数和氮含量）方面表现更有效，这在600 mg kg−1镍处理下的生理特性和抗氧化酶中得到体现。在第二个季节，即2020/2021年，观察到类似模式。此外，在蚕豆植株（根和地上部分）中发现镍浓度较低。因此，耐受性最强的根瘤菌（TAL−1148）+枯草芽孢杆菌处理组合可用于降低镍毒性。
