Arafa Ramadan A, Kamel Said M, Taher Dalia I, Solberg Svein Ø, Rakha Mohamed T
Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt.
Vegetable Crops Research Department, Horticulture Research Institute, Agriculture Research Center, Giza 12619, Egypt.
Plants (Basel). 2022 Jul 12;11(14):1824. doi: 10.3390/plants11141824.
Late blight disease, caused by (Mont.) de Bary, is one of the most challenging diseases threatening tomato production and other Solanaceae crops. Resistance to late blight is found in certain wild species, but the mechanism behind the resistance is not fully understood. The aim of this study was to examine the metabolic profiles in the leaf tissue of late blight-resistant wild tomato and to investigate if leaf extracts from such genotypes could be used to control late blight in tomato production. We included three recognized late blight-resistant wild tomato accessions of (LA1777, LA2855, and LA1352) and two recognized highly susceptible genotypes, ('Super Strain B') and (LA0375). The metabolic profiles were obtained in both inoculated and non-inoculated plants by analyzing leaf extracts using high-resolution gas chromatography-mass spectrometry (GC-MS) with three replicate analyses of each genotype. We focused on volatile organic compounds (VOCs) and identified 31 such compounds from the five genotypes with a retention time ranging from 6.6 to 22.8 min. The resistant genotype LA 1777 produced the highest number of VOCs (22 and 21 in the inoculated and control plants, respectively), whereas the susceptible genotype 'Super Strain B' produced the lowest number of VOCs (11 and 13 in the respective plants). Among the VOCs, 14 were detected only in the resistant genotypes, while two were detected only in the susceptible ones. In vitro trials, with the use of a detached leaflet assay and whole-plant approach, were conducted. We revealed promising insights regarding late blight management and showed that metabolic profiling may contribute to a better understanding of the mechanisms behind resistance in tomato and its wild relatives.
由致病疫霉（Mont.）德巴里引起的晚疫病是威胁番茄生产和其他茄科作物的最具挑战性的病害之一。在某些野生种中发现了对晚疫病的抗性，但其抗性背后的机制尚未完全了解。本研究的目的是检测抗晚疫病野生番茄叶片组织中的代谢谱，并研究此类基因型的叶片提取物是否可用于控制番茄生产中的晚疫病。我们纳入了三个公认的抗晚疫病野生番茄种质（LA1777、LA2855和LA1352）以及两个公认的高感基因型（“超级菌株B”和LA0375）。通过使用高分辨率气相色谱 - 质谱联用仪（GC - MS）分析叶片提取物，对接种和未接种的植株均进行了代谢谱分析，每个基因型进行三次重复分析。我们重点关注挥发性有机化合物（VOCs），从这五个基因型中鉴定出31种此类化合物，保留时间范围为6.6至22.8分钟。抗性基因型LA 1777产生的VOCs数量最多（接种和对照植株中分别为22种和21种），而感病基因型“超级菌株B”产生的VOCs数量最少（相应植株中分别为11种和13种）。在VOCs中，14种仅在抗性基因型中检测到，而2种仅在感病基因型中检测到。进行了离体叶片试验和整株试验，采用了离体小叶测定法和整株试验方法。我们揭示了关于晚疫病管理的有前景的见解，并表明代谢谱分析可能有助于更好地理解番茄及其野生近缘种中抗晚疫病的机制。
