Šćepanović Maja, Košćak Laura, Pismarović Laura, Šoštarčić Valentina
Department of Weed Science, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia.
Department of Agriculture and Nutrition, Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, Croatia.
Plants (Basel). 2022 Jul 20;11(14):1888. doi: 10.3390/plants11141888.
Herbicides are the most commonly used means of controlling the growth of L. Their constant use has led to the development of resistant populations. They can be evaluated by studying seed germination and the corresponding grown plants, but exhibits seed dormancy, preventing germination and delaying research. Here, we developed a simple and rapid method to stimulate germination of freshly collected or stored seeds. The germination of freshly collected/stored seeds was evaluated after storage, stratification, and chemical treatments (ethephon, gibberellic acid (GA), thiourea, KNO). Ethephon or ethephon + GA improved freshly collected seed germination by 88 and 95%, respectively, and germination of stored seeds by 78 and 80%, respectively. In addition, placing the seeds of in ethephon, GA, ethephon + GA, or thiourea solutions caused the freshly collected seeds to germinate faster than stored seeds or nontreated seeds. In contrast, the conditioning of seeds in these solutions favored germination of stored seeds, especially when ethephon + GA or GA was used. Imbibition of the freshly collected seeds in a mixture of ethephon and GA can effectively overcome primary dormancy when rapid experimental results are needed. For seeds requiring prolonged storage, conditioning in ethephon, GA, or thiourea solutions may be applied to promote germination.
除草剂是控制L生长最常用的手段。其持续使用导致了抗性种群的发展。可以通过研究种子萌发和相应的生长植株来评估它们，但L表现出种子休眠，这会阻止萌发并延迟研究。在此，我们开发了一种简单快速的方法来刺激新鲜采集或储存的L种子萌发。在储存、层积处理和化学处理（乙烯利、赤霉素（GA）、硫脲、硝酸钾）后，对新鲜采集/储存的L种子的萌发情况进行了评估。乙烯利或乙烯利 + GA分别使新鲜采集种子的萌发率提高了88%和95%，使储存种子的萌发率分别提高了78%和80%。此外，将L种子置于乙烯利、GA、乙烯利 + GA或硫脲溶液中，会使新鲜采集的种子比储存种子或未处理种子萌发得更快。相比之下，将种子置于这些溶液中进行预处理有利于储存种子的萌发，尤其是使用乙烯利 + GA或GA时。当需要快速得到实验结果时，将新鲜采集的L种子浸泡在乙烯利和GA的混合物中可以有效克服初始休眠。对于需要长时间储存的种子，可以采用在乙烯利、GA或硫脲溶液中进行预处理的方法来促进萌发。
