Prijić Željana, Mikić Sara, Peškanov Jovan, Zhang Xiuxin, Guo Lili, Dragumilo Ana, Filipović Vladimir, Anačkov Goran, Marković Tatjana
Institute for Medicinal Plants Research "D Josif Pančić" Belgrade, Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.
Plants (Basel). 2024 Aug 6;13(16):2178. doi: 10.3390/plants13162178.
Mill. is a protected, herbaceous species native to Southeastern Europe and Turkey. Due to its vulnerability, it has to be protected both in its natural habitats and through cultivation. Peonies are known to have a low potential for natural regeneration due to their seed dormancy, low germination rate, and long germination period. In this study, treatments with gibberellic acid (GA 150, 200, 250, 300, and 350 mg L GA) and warm (at 20/16 °C day/night regime) and cold stratification (at 4 °C) were used to accelerate dormancy release and increase the germination rate. The seeds of from four natural habitats in Serbia and the Institute's collection were collected and analyzed. They showed an underdeveloped embryo that needs to grow inside the seed before it can germinate. The application of GA accelerated each stage of germination (seed coat rapturing, hypocotyl dormancy release, and epicotyl dormancy release) for approximately 10 days compared to the control. It was also found that two-day imbibition with 200 mg L GA significantly accelerated and equalized germination. Higher GA concentrations had a more pronounced impact on each stage but also resulted in greater seed infection after the seed coat rupture, elongated and weak seedlings, while lower concentrations did not result in obtaining uniform seedlings. There were no significant differences observed between localities. Restoring through seeds and nursery-produced plants is crucial for conserving the genetic diversity of the tested species.
Mill. 是一种原产于东南欧和土耳其的受保护草本物种。由于其易危性，必须在其自然栖息地以及通过人工栽培进行保护。已知牡丹由于种子休眠、发芽率低和发芽期长，自然更新潜力较低。在本研究中，使用赤霉素（GA 150、200、250、300和350毫克/升GA）处理以及温暖（白天/夜间温度为20/16°C）和冷层积（4°C）处理来加速休眠解除并提高发芽率。收集并分析了来自塞尔维亚四个自然栖息地以及该研究所种质库的种子。这些种子显示出胚发育不全，在发芽前需要在种子内部生长。与对照相比，GA的应用使发芽的各个阶段（种皮破裂、下胚轴休眠解除和上胚轴休眠解除）提前了约10天。还发现用200毫克/升GA浸种两天可显著加速并使发芽均匀。较高浓度的GA对每个阶段的影响更明显，但也导致种皮破裂后种子感染增加、幼苗细长且脆弱，而较低浓度则无法获得均匀的幼苗。不同产地之间未观察到显著差异。通过种子和苗圃培育植物来恢复该物种对于保护受试物种的遗传多样性至关重要。
