de Lima Tielidy A de M, de Lima Gabriel Goetten, Chee Bor Shin, Henn Jeferson G, Cortese Yvonne J, Matos Mailson, Helm Cristiane V, Magalhães Washington L E, Nugent Michael J D
Materials Research Institute, Technological University of the Shannon: Midlands Midwest, N37HD68 Athlone, Ireland.
Programa de Pós-Graduação em Engenharia e Ciência dos Materiais-PIPE, Universidade Federal do Paraná, Curitiba 81531-980, Brazil.
Polymers (Basel). 2022 Jul 7;14(14):2776. doi: 10.3390/polym14142776.
The reuse of agro-industrial waste assumes great importance today. Pinhão is the seed of , which is native to the mountains of southern Brazil, Paraguay, and Argentina. The coat is a by-product of this seed and is rich in phenolic compounds. The present study aimed to use the residue as a precursor material for the production of nanocellulose through the mechanical defibrillation process and perform the characterization of the films and the gel to investigate the effect on the physical and regenerative properties when incorporated with polyvinyl alcohol (PVA). The modulus of elasticity was higher when the MFC of pinhão was added to the PVA. Film and gel had their cytotoxicity tested by MTT assay using 3T3 fibroblast and Schwann cancer cells, and a migration assay was also performed using the scratch test on HaCat keratinocyte cells. For the scratch test, film and gel samples with low concentration presented a complete scratch closure in 72 h. Molecular docking was performed and quercetin had the ideal interaction score values, so it was used with the PACAP protein which presented a slightly moderate interaction with the protein synthesis of Schwann cells, presenting compactness of the compound after 14 ns.
如今，农业工业废弃物的再利用具有重要意义。松子是[某种植物]的种子，原产于巴西南部、巴拉圭和阿根廷的山区。松子壳是这种种子的副产品，富含酚类化合物。本研究旨在将该残渣用作通过机械去纤颤法生产纳米纤维素的前驱体材料，并对薄膜和凝胶进行表征，以研究其与聚乙烯醇（PVA）混合时对物理和再生性能的影响。当将松子微晶纤维素（MFC）添加到PVA中时，弹性模量更高。使用3T3成纤维细胞和施万癌细胞通过MTT法对薄膜和凝胶进行细胞毒性测试，并使用划痕试验对HaCat角质形成细胞进行迁移试验。对于划痕试验，低浓度的薄膜和凝胶样品在72小时内完全闭合划痕。进行了分子对接，槲皮素具有理想的相互作用得分值，因此将其与垂体腺苷酸环化酶激活肽（PACAP）蛋白一起使用，该蛋白与施万细胞的蛋白质合成呈现出中等程度的相互作用，在14纳秒后呈现出化合物的紧密性。
