Bagwe Priyal, Bajaj Lotika, Gala Rikhav P, D'Souza Martin J, Zughaier Susu M
Vaccine Nanotechnology Laboratory, Center for Drug Delivery Research, College of Pharmacy, Mercer University, Atlanta, GA 30341, USA.
Fraunhofer USA, Center Mid-Atlantic, Biotechnology Division, 9, Innovation Way, Newark, DE 19011, USA.
Vaccines (Basel). 2022 Jun 21;10(7):983. doi: 10.3390/vaccines10070983.
The emergence of drug-resistant gonorrhea infections worldwide combined with the lack of a vaccine is alarming. We prepared a novel microparticulate (MP) vaccine formulation using whole-cell inactivated as the vaccine antigen, with Alum and AddaVax™ as vaccine adjuvants. The adjuvanted vaccine MP formulation was assessed for in vitro immunostimulatory activity, autophagy, and antigen presentation ability. The data shows that the adjuvanted gonococci vaccine MP enhanced autophagy induction in antigen presenting cells (APCs) compared to gonococci vaccine MP without adjuvants, which is important for enhancing antigen presentation. In addition, the adjuvanted vaccine formulation increased the surface expression of antigen presenting molecules MHCI and MHCII as well as co-stimulatory molecules CD40 and CD86 on the surface of dendritic cells. In addition, the gonococci vaccine microparticles at lower doses did not significantly increase the expression of the death receptor CD95 in APCs, which when elevated leads to suboptimal antigen presentation and reduced immune responses. The adjuvanted whole-cell inactivated gonococci microparticle vaccine formulation enhanced antigen uptake, processing, and antigen presentation.
全球范围内耐药性淋病感染的出现，再加上缺乏疫苗，令人担忧。我们制备了一种新型微粒（MP）疫苗制剂，使用全细胞灭活物作为疫苗抗原，以明矾和AddaVax™作为疫苗佐剂。对佐剂化疫苗MP制剂进行了体外免疫刺激活性、自噬和抗原呈递能力评估。数据表明，与无佐剂的淋球菌疫苗MP相比，佐剂化淋球菌疫苗MP增强了抗原呈递细胞（APC）中的自噬诱导，这对于增强抗原呈递很重要。此外，佐剂化疫苗制剂增加了树突状细胞表面抗原呈递分子MHCI和MHCII以及共刺激分子CD40和CD86的表面表达。此外，较低剂量的淋球菌疫苗微粒不会显著增加APC中死亡受体CD95的表达，而CD95表达升高会导致抗原呈递不理想和免疫反应降低。佐剂化全细胞灭活淋球菌微粒疫苗制剂增强了抗原摄取、加工和抗原呈递。
