Forouharmehr A, Banan A, Mousavi S M, Jaydari A
Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
Department of Marine Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
Arch Razi Inst. 2022 Feb 28;77(1):45-56. doi: 10.22092/ARI.2021.353377.1601. eCollection 2022 Feb.
infection is recognized as a disease with substantial economic losses, infecting a wide range of fish species. The limitations of current vaccines and strategies have led to the identification of new methods to control this disease. Multi-epitope vaccines which employ various immunogenic proteins can be promising. The current research project aimed to design an efficient multi-epitope vaccine against infection in fish. To this end, six immunogenic proteins of , including FBA, ENO, Sip11, GAPDH, MtsB, and SCPI proteins, were applied for epitope prediction. The best B cell, T cell, and IFNγ epitopes of the immunogenic proteins, as well as interleukin-8, were used to construct a multi-epitope vaccine. Thereafter, different parameters of the designed vaccine, including physicochemical features, antigenicity, secondary structure, and tertiary structure, were evaluated. Moreover, the interaction of the interleukin-8 domain of the designed vaccine and its receptor was investigated by molecular docking strategy. Finally, nucleotide sequence of the vaccine was adapted to express in . The results of the present study pointed out that the designed vaccine was a stable vaccine with molecular weight and antigenicity score of 45 kDa and 0.936, respectively. Furthermore, the structure analysis results revealed that the designed vaccine contained 23.49% alpha helix, with 90.5% residues in favored region. Finally, it was demonstrated that the interleukin-8 domain of the designed vaccine could be successfully docked to its receptor with the lowest energy of -1020.9. Based on the obtained results, it seems that the designed vaccine can be an efficient candidate to prevent infection in fish.
感染被认为是一种造成重大经济损失的疾病，可感染多种鱼类。当前疫苗和策略的局限性促使人们寻找控制这种疾病的新方法。采用多种免疫原性蛋白的多表位疫苗可能具有前景。当前的研究项目旨在设计一种高效的抗鱼类感染多表位疫苗。为此，应用了该病原体的六种免疫原性蛋白，包括FBA、ENO、Sip11、GAPDH、MtsB和SCPI蛋白进行表位预测。利用免疫原性蛋白的最佳B细胞、T细胞和IFNγ表位以及白细胞介素-8来构建一种多表位疫苗。此后，评估了所设计疫苗的不同参数，包括物理化学特性、抗原性、二级结构和三级结构。此外，通过分子对接策略研究了所设计疫苗的白细胞介素-8结构域与其受体的相互作用。最后，对疫苗的核苷酸序列进行改造以便在（此处原文缺失具体表达系统）中表达。本研究结果指出，所设计的疫苗是一种稳定的疫苗，分子量为45 kDa，抗原性评分为0.936。此外，结构分析结果显示，所设计的疫苗含有23.49%的α螺旋，90.5%的残基处于有利区域。最后，证明所设计疫苗的白细胞介素-8结构域能够以-1020.9的最低能量成功对接至其受体。基于所得结果，所设计的疫苗似乎可以成为预防鱼类感染的有效候选疫苗。
