Hajissa Khalid, Zakaria Robaiza, Suppian Rapeah, Mohamed Zeehaida
Dept. of Zoology, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan.
Dept. of Medical Microbiology & Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Ku-bang Kerian, Kelantan, Malaysia.
Iran J Parasitol. 2018 Apr-Jun;13(2):215-224.
is a widely prevalent intracellular protozoan parasite which causes serious clinical and veterinary problems. Development of an effective vaccine for controlling toxoplasmosis is an extremely important aim. In the present study, the protective efficacy of recombinant multiepitope antigen (USM.TOXO1) expressing nine potential epitopes identified from SAG1, GRA2, and GRA7 of was evaluated in BALB/c mice.
Mice were immunized subcutaneously with three doses of USM.TOXO1 antigen (10 μg/ml). Following the immunization, the IgG antibody, IgG subclass, IFN-γ and IL-4 production were evaluated using ELISA, the study was conducted at Animal Research and Service Center (ARASC), USM Health Campus in 2016.
Mice immunized with USM.TOXO1 significantly induced a mixed Th1/Th2 response polarized toward the IgG1 antibody isotype. While the cytokine analysis revealed a significant release of IFN-γ cytokines.
USM.TOXO1 is a potential vaccine candidate that elicits strong immunity in BALB/c mice. The proven immunogenicity of the generated antigen can serve as a premise for further use of epitope-based vaccine in the immunoprevention of human and animal toxoplasmosis.
是一种广泛流行的细胞内原生动物寄生虫，可引发严重的临床和兽医问题。开发一种有效的控制弓形虫病的疫苗是一个极其重要的目标。在本研究中，在BALB/c小鼠中评估了表达从弓形虫SAG1、GRA2和GRA7中鉴定出的九个潜在表位的重组多表位抗原（USM.TOXO1）的保护效力。
用三剂USM.TOXO1抗原（10μg/ml）对小鼠进行皮下免疫。免疫后，使用ELISA评估IgG抗体、IgG亚类、IFN-γ和IL-4的产生，该研究于2016年在美国玛拉工艺大学健康校区动物研究与服务中心（ARASC）进行。
用USM.TOXO1免疫的小鼠显著诱导了偏向IgG1抗体亚型的混合Th1/Th2反应。而细胞因子分析显示IFN-γ细胞因子有显著释放。
USM.TOXO1是一种潜在的疫苗候选物，可在BALB/c小鼠中引发强大的免疫力。所产生抗原已证实的免疫原性可作为进一步将基于表位的疫苗用于人和动物弓形虫病免疫预防的前提。
