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从烧伤和创伤感染中分离的抗生素耐药基因研究。

Study of Antibiotic Resistant Genes in Isolated from Burns and Wounds.

机构信息

Microbiology Department, School of Medicine, University of Kirkuk, Kirkuk, Iraq.

出版信息

Arch Razi Inst. 2022 Feb 28;77(1):403-411. doi: 10.22092/ARI.2021.356681.1893. eCollection 2022 Feb.

Abstract

() is frequently associated with infections with high mortality rates. The intrinsically high resistance to many antibiotics and multidrug resistance in the hospital setting is considered to be among the reasons for high pathogenicity of . In this study, a total of 200 wound and burn swabs were collected from patients. The collected specimens were examined for through biochemical and antibacterial sensitivity tests performed in the Microbiology Laboratory in College of Medicine, University of Kirkuk, Kirkuk, Iraq. The polymerase chain reaction was then used to detect mexA, mexB, mexR, and oprD genes. In total, 31 isolates of were collected from 200 patients with wounds and burns. Most cases were isolated from 23 (74.19%) and 8 (25.80%) wound and burn swabs, respectively. Antibiotic sensitivity was tested on all isolates against 17 antimicrobial agents. The obtained results revealed a high resistance rate to gentamicin, trimethoprim, amikacin, and amoxicillin, and a low resistance rate was observed to ceftazidime, tobramycin, levofloxacin, cotrimoxazole, ciprofloxacin, and aztreonam. Regarding antibiotic resistance, mexB, mexR, and oprD genes were observed in three isolates, in which mexB and mexR were detected in two isolates, and only one isolate carried mexA gene.

摘要

()常与高死亡率的感染有关。在医院环境中,对许多抗生素具有固有抗性和多药耐药性被认为是高致病性的原因之一。在这项研究中,从患者身上采集了 200 份伤口和烧伤拭子。在伊拉克基尔库克大学医学院微生物实验室进行的生化和抗菌敏感性测试中,对收集的标本进行了 检测。然后使用聚合酶链反应检测 mexA、mexB、mexR 和 oprD 基因。总共从 200 名患有伤口和烧伤的患者中收集了 31 株 。大多数病例分别从 23 个(74.19%)和 8 个(25.80%)伤口和烧伤拭子中分离出来。对所有分离株进行了 17 种抗菌药物的敏感性测试。结果显示,所有分离株对庆大霉素、甲氧苄啶、阿米卡星和阿莫西林的耐药率较高,对头孢他啶、妥布霉素、左氧氟沙星、复方新诺明、环丙沙星和氨曲南的耐药率较低。关于抗生素耐药性,在三个分离株中观察到了 mexB、mexR 和 oprD 基因,其中在两个分离株中检测到了 mexB 和 mexR,而只有一个分离株携带了 mexA 基因。

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