Department of Biology, College of Sciences, University of Babylon, Babylon, Iraq.
Department of Medicine, Neurology, College of Medicine, University of Babylon, Babylon, Iraq.
Arch Razi Inst. 2022 Feb 28;77(1):81-86. doi: 10.22092/ARI.2021.356470.1848. eCollection 2022 Feb.
Multiple sclerosis (MS) is a chronic autoimmune disorder of the central nervous system. Genetic and environmental factors have important roles in the induction, onset, and progression of MS. In this study, the IL-21 genotype (rs2055979) (G/T) in Iraqi MS patients was compared with a healthy control group to investigate the possible association of any particular genotype or allele with multiple sclerosis. This study included 70 patients with relapsing-remitting MS and 50 healthy individuals as control. Following the extraction of genomic DNA, polymerase chain reaction-restriction fragment length polymorphism, the frequencies of genotypes, and alleles were calculated and statistically analyzed. The results of the study revealed a significant reduction in the distribution of the wild homozygous genotype (GG) in MS patients, in comparison to a healthy control group (14.3% vs. 34 %; 0.0129 at P≤0.05; odds ratio [OR] 3.0909, 95% confidence interval [CI]: 1.2704-7.5203). However, MS cases and controls did not differ significantly in neither GT nor TT genotypes, 62.9% (OR 0.6402, 95% CI: 0.3064-1.3374) and 52% (OR 0.5494, 95% CI: 0.2074-1.4557), respectively. The data of allele frequencies in patients and controls showed that the G allele frequencies were 0.46 vs. 0.60 in patients and controls, respectively, while T allele frequencies were 0.54 vs. 0.40 in patients and controls, respectively. The current conclusions indicated that in the study group, the GG genotype of IL-21(rs2055979) could be related to MS.
多发性硬化症（MS）是一种中枢神经系统的慢性自身免疫性疾病。遗传和环境因素在 MS 的诱导、发病和进展中起着重要作用。在这项研究中，比较了伊拉克 MS 患者的 IL-21 基因型（rs2055979）（G/T）与健康对照组，以研究任何特定基因型或等位基因与多发性硬化症的可能关联。这项研究包括 70 例复发缓解型 MS 患者和 50 名健康对照者。提取基因组 DNA 后，采用聚合酶链反应-限制性片段长度多态性分析，计算并统计分析基因型和等位基因的频率。研究结果显示，与健康对照组相比，MS 患者野生纯合基因型（GG）的分布显著减少（14.3%对 34%；0.0129，P≤0.05；比值比[OR]3.0909，95%置信区间[CI]：1.2704-7.5203）。然而，MS 病例和对照组在 GT 或 TT 基因型中均无显著差异，分别为 62.9%（OR 0.6402，95%CI：0.3064-1.3374）和 52%（OR 0.5494，95%CI：0.2074-1.4557）。患者和对照组的等位基因频率数据显示，G 等位基因频率分别为 0.46 和 0.60，T 等位基因频率分别为 0.54 和 0.40。目前的结论表明，在研究组中，IL-21(rs2055979)的 GG 基因型可能与 MS 有关。
