Wu Shi Min, Chen Juan, Liang Ying, Luo Qian, Tong Yao Yao, Xie Ling
Center for Clinical Laboratory, General Hospital of The Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, Hubei, China. Email:
Center for Clinical Laboratory, General Hospital of The Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, Hubei, China.
Cell J. 2022 Jun;24(6):285-293. doi: 10.22074/cellj.2022.7963. Epub 2022 Jun 29.
Long non-coding RNAs (lncRNAs) feature prominently in tumors. Reportedly, lncRNA zinc finger E-box-binding homeobox 2 antisense RNA 1 (ZEB2-AS1) is aberrantly expressed in a variety of tumors. The present study was aimed to explore ZEB2-AS1 functions and determine mechanism in hepatocellular carcinoma (HCC) progression.
In this experimental study, expressions of , microRNA and forkhead box C1 mRNA in HCC tissues and cell lines were detected via quantitative reveres transcription polymerase chain reaction (qRT-PCR). After establishing gain- and loss-of-functions models, cell counting kit-8, 5-bromo-2'-deoxyuridine (BrdU), Transwell assays and flow cytometry analysis were conducted to examine HCC cell multiplication, migration, invasion and apoptosis, respectively. The targeted relationship between and was verified via dual-luciferase reporter gene assay. Western blot was utilized for detecting FOXC1 expression in HCC cells after selectively regulating and
In HCC tissues and cells, expression was increased. High ZEB2-AS1 expression was related to relatively large tumor volume, increased tumor-node-metastasis (TNM) stage and positive lymph node metastasis of the patients. overexpression facilitated HCC cell multiplication, migration, invasion and suppressed apoptosis, while knock-down caused the opposite effects. It was also confirmed that could competitively bind with to repress its expression, and indirectly up-regulate FOXC1 expression level in HCC cells.
The current study revealed that was over-expressed in HCC tissues and cells. It also upregulated , through sponging , to promote HCC progression. This provides new perspectives for elucidating the pathogenesis of HCC.
长链非编码RNA（lncRNAs）在肿瘤中起着重要作用。据报道，lncRNA锌指E盒结合同源框2反义RNA 1（ZEB2-AS1）在多种肿瘤中异常表达。本研究旨在探讨ZEB2-AS1在肝细胞癌（HCC）进展中的功能并确定其机制。
在本实验研究中，通过定量逆转录聚合酶链反应（qRT-PCR）检测HCC组织和细胞系中、微小RNA和叉头框C1（FOXC1）mRNA的表达。建立功能获得和功能缺失模型后，分别进行细胞计数试剂盒-8、5-溴-2'-脱氧尿苷（BrdU）、Transwell实验和流式细胞术分析，以检测HCC细胞的增殖、迁移、侵袭和凋亡。通过双荧光素酶报告基因实验验证与之间的靶向关系。在选择性调节和后，利用蛋白质免疫印迹法检测HCC细胞中FOXC1的表达。
在HCC组织和细胞中，表达增加。ZEB2-AS1高表达与患者相对较大的肿瘤体积、肿瘤-淋巴结-转移（TNM）分期增加和阳性淋巴结转移有关。过表达促进HCC细胞增殖、迁移、侵袭并抑制凋亡，而敲低则产生相反的效果。还证实可以与竞争性结合以抑制其表达，并间接上调HCC细胞中FOXC1的表达水平。
当前研究表明在HCC组织和细胞中过表达。它还通过海绵吸附上调，以促进HCC进展。这为阐明HCC的发病机制提供了新的视角。
