• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码 RNA CCDC183-AS1 通过调控 SKP1 表达作为 miR-589-5p 的海绵促进肝癌的进展。

Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.

机构信息

Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.

Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, P.R. China.

出版信息

J Exp Clin Cancer Res. 2021 Feb 4;40(1):57. doi: 10.1186/s13046-021-01861-6.

DOI:10.1186/s13046-021-01861-6
PMID:33541391
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7863448/
Abstract

BACKGROUND

Hepatocellular carcinoma (HCC) is a common type of malignant human cancer with high morbidity and poor prognosis, causing numerous deaths per year worldwide. Growing evidence has been demonstrated that long non-coding RNAs (lncRNAs) are closely associated with hepatocarcinogenesis and metastasis. However, the roles, functions, and working mechanisms of most lncRNAs in HCC remain poorly defined.

METHODS

Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of CCDC183-AS1 in HCC tissues and cell lines. Cell proliferation, migration and invasion ability were evaluated by CCK-8 and transwell assay, respectively. Animal experiments were used to explore the role of CCDC183-AS1 and miR-589-5p in vivo. Bioinformatic analysis, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were performed to confirm the regulatory relationship between CCDC183-AS1, miR-589-5p and SKP1.

RESULTS

Significantly upregulated expression of CCDC183-AS1 was observed in both HCC tissues and cell lines. HCC patients with higher expression of CCDC183-AS1 had a poorer overall survival rate. Functionally, overexpression of CCDC183-AS1 markedly promoted HCC cell proliferation, migration and invasion in vitro and tumor growth and metastasis in vivo, whereas the downregulation of CCDC183-AS1 exerted opposite effects. MiR-589-5p inhibitor counteracted the proliferation, migration and invasion inhibitory effects induced by CCDC183-AS1 silencing. Mechanistically, CCDC183-AS1 acted as a ceRNA through sponging miR-589-5p to offset its inhibitory effect on the target gene SKP1, then promoted the tumorigenesis of HCC.

CONCLUSIONS

CCDC183-AS1 functions as an oncogene to promote HCC progression through the CCDC183-AS1/miR-589-5p/SKP1 axis. Our study provided a novel potential therapeutic target for HCC patients.

摘要

背景

肝细胞癌（HCC）是一种常见的人类恶性肿瘤，发病率高，预后差，每年在全球范围内导致大量死亡。越来越多的证据表明，长链非编码 RNA（lncRNA）与肝癌的发生和转移密切相关。然而，大多数 lncRNA 在 HCC 中的作用、功能和作用机制仍未得到明确界定。

方法

实时定量聚合酶链反应（qRT-PCR）检测 HCC 组织和细胞系中 CCDC183-AS1 的表达水平。CCK-8 法和 Transwell 实验分别评估细胞增殖、迁移和侵袭能力。动物实验用于研究 CCDC183-AS1 和 miR-589-5p 在体内的作用。生物信息学分析、双荧光素酶报告基因实验和 RNA 免疫沉淀（RIP）实验证实了 CCDC183-AS1、miR-589-5p 和 SKP1 之间的调控关系。

结果

CCDC183-AS1 在 HCC 组织和细胞系中均呈显著上调表达。CCDC183-AS1 表达较高的 HCC 患者总生存率较低。功能上，CCDC183-AS1 过表达显著促进 HCC 细胞体外增殖、迁移和侵袭，体内肿瘤生长和转移，而 CCDC183-AS1 下调则产生相反的效果。CCDC183-AS1 沉默诱导的增殖、迁移和侵袭抑制作用被 miR-589-5p 抑制剂逆转。机制上，CCDC183-AS1 通过海绵吸附 miR-589-5p 作为 ceRNA 来抵消其对靶基因 SKP1 的抑制作用，从而促进 HCC 的发生。

结论

CCDC183-AS1 通过 CCDC183-AS1/miR-589-5p/SKP1 轴发挥癌基因作用，促进 HCC 进展。本研究为 HCC 患者提供了一个新的潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/2834082f9ab1/13046_2021_1861_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/fadeeb12af78/13046_2021_1861_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/77636f727022/13046_2021_1861_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/5871f12f9d61/13046_2021_1861_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/460d7ba9be32/13046_2021_1861_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/455528f5ff0e/13046_2021_1861_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/080d658c6219/13046_2021_1861_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/2834082f9ab1/13046_2021_1861_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/fadeeb12af78/13046_2021_1861_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/77636f727022/13046_2021_1861_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/5871f12f9d61/13046_2021_1861_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/460d7ba9be32/13046_2021_1861_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/455528f5ff0e/13046_2021_1861_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/080d658c6219/13046_2021_1861_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7376/7863448/2834082f9ab1/13046_2021_1861_Fig7_HTML.jpg

相似文献

1
Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.长链非编码 RNA CCDC183-AS1 通过调控 SKP1 表达作为 miR-589-5p 的海绵促进肝癌的进展。
J Exp Clin Cancer Res. 2021 Feb 4;40(1):57. doi: 10.1186/s13046-021-01861-6.
2
Long Noncoding RNA TRIM52-AS1 Sponges miR-514a-5p to Facilitate Hepatocellular Carcinoma Progression Through Increasing MRPS18A.长链非编码 RNA TRIM52-AS1 通过海绵吸附 miR-514a-5p 促进肝细胞癌进展
Cancer Biother Radiopharm. 2021 Mar;36(2):211-219. doi: 10.1089/cbr.2019.3271. Epub 2020 May 8.
3
Long non-coding RNA MAPKAPK5-AS1/PLAGL2/HIF-1α signaling loop promotes hepatocellular carcinoma progression.长非编码 RNA MAPKAPK5-AS1/PLAGL2/HIF-1α 信号通路促进肝细胞癌进展。
J Exp Clin Cancer Res. 2021 Feb 17;40(1):72. doi: 10.1186/s13046-021-01868-z.
4
Long non-coding RNA AGAP2-AS1, functioning as a competitive endogenous RNA, upregulates ANXA11 expression by sponging miR-16-5p and promotes proliferation and metastasis in hepatocellular carcinoma.长链非编码 RNA AGAP2-AS1 作为竞争性内源性 RNA，通过海绵吸附 miR-16-5p 而上调 ANXA11 的表达，促进肝癌的增殖和转移。
J Exp Clin Cancer Res. 2019 May 14;38(1):194. doi: 10.1186/s13046-019-1188-x.
5
A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis.一种新型长链非编码 RNA MCM3AP-AS1 通过靶向 miR-194-5p/FOXA1 轴促进肝癌的生长。
Mol Cancer. 2019 Feb 19;18(1):28. doi: 10.1186/s12943-019-0957-7.
6
Long non-coding RNA SBF2-AS1 promotes hepatocellular carcinoma progression through regulation of miR-140-5p-TGFBR1 pathway.长链非编码 RNA SBF2-AS1 通过调控 miR-140-5p-TGFBR1 通路促进肝癌进展。
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2826-2832. doi: 10.1016/j.bbrc.2018.08.047. Epub 2018 Aug 14.
7
RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin β5 axis.环状 RNA RNF185-AS1 通过靶向 miR-221-5p/整合素 β5 轴促进肝癌进展。
Life Sci. 2021 Feb 15;267:118928. doi: 10.1016/j.lfs.2020.118928. Epub 2020 Dec 31.
8
LncRNA MYLK-AS1 facilitates tumor progression and angiogenesis by targeting miR-424-5p/E2F7 axis and activating VEGFR-2 signaling pathway in hepatocellular carcinoma.长链非编码 RNA MYLK-AS1 通过靶向 miR-424-5p/E2F7 轴和激活血管内皮生长因子受体 2 信号通路促进肝癌的进展和血管生成。
J Exp Clin Cancer Res. 2020 Nov 9;39(1):235. doi: 10.1186/s13046-020-01739-z.
9
Long noncoding RNA UPK1A-AS1 indicates poor prognosis of hepatocellular carcinoma and promotes cell proliferation through interaction with EZH2.长链非编码 RNA UPK1A-AS1 提示肝细胞癌预后不良，并通过与 EZH2 相互作用促进细胞增殖。
J Exp Clin Cancer Res. 2020 Oct 29;39(1):229. doi: 10.1186/s13046-020-01748-y.
10
Knockdown of LncRNA ANRIL suppresses cell proliferation, metastasis, and invasion via regulating miR-122-5p expression in hepatocellular carcinoma.长链非编码RNA ANRIL的敲低通过调节miR-122-5p的表达抑制肝癌细胞的增殖、转移和侵袭。
J Cancer Res Clin Oncol. 2018 Feb;144(2):205-214. doi: 10.1007/s00432-017-2543-y. Epub 2017 Nov 10.

引用本文的文献

1
Research Progress with Atractylone as an Antitumor Agent.白术抗肿瘤作用的研究进展。
Molecules. 2024 Nov 19;29(22):5450. doi: 10.3390/molecules29225450.
2
Prognostic signature and immune landscape of 5-methylcytosine-related long non-coding RNAs in gastric cancer.胃癌中5-甲基胞嘧啶相关长链非编码RNA的预后特征及免疫图谱
Heliyon. 2024 Sep 14;10(18):e37290. doi: 10.1016/j.heliyon.2024.e37290. eCollection 2024 Sep 30.
3
TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling.

本文引用的文献

1
Ubiquitin signaling in cell cycle control and tumorigenesis.泛素信号在细胞周期调控和肿瘤发生中的作用。
Cell Death Differ. 2021 Feb;28(2):427-438. doi: 10.1038/s41418-020-00648-0. Epub 2020 Oct 31.
2
AFP promotes HCC progression by suppressing the HuR-mediated Fas/FADD apoptotic pathway.AFP 通过抑制 HuR 介导的 Fas/FADD 凋亡途径促进 HCC 进展。
Cell Death Dis. 2020 Oct 2;11(10):822. doi: 10.1038/s41419-020-03030-7.
3
Regulation of cell cycle drivers by Cullin-RING ubiquitin ligases.Cullin-RING 泛素连接酶对细胞周期驱动因子的调控。
TRIM55通过稳定TRIP6激活Wnt/β-连环蛋白信号促进肝细胞癌增殖。
J Hepatocell Carcinoma. 2023 Aug 3;10:1281-1293. doi: 10.2147/JHC.S418049. eCollection 2023.
4
Long noncoding RNA CCDC183-AS1 depletion represses breast cancer cell proliferation, colony formation, and motility by sponging microRNA-3918.长链非编码 RNA CCDC183-AS1 通过海绵吸附 microRNA-3918 抑制乳腺癌细胞增殖、集落形成和迁移。
Oncol Res. 2022 Aug 1;29(3):189-200. doi: 10.32604/or.2022.03573. eCollection 2021.
5
An in vitro approach to understand contribution of kidney cells to human urinary extracellular vesicles.体外方法研究肾脏细胞对人尿细胞外囊泡的贡献。
J Extracell Vesicles. 2023 Feb;12(2):e12304. doi: 10.1002/jev2.12304.
6
Exosome-Delivered circSTAU2 Inhibits the Progression of Gastric Cancer by Targeting the miR-589/CAPZA1 Axis.外泌体递送的 circSTAU2 通过靶向 miR-589/CAPZA1 轴抑制胃癌的进展。
Int J Nanomedicine. 2023 Jan 6;18:127-142. doi: 10.2147/IJN.S391872. eCollection 2023.
7
A micropeptide JunBP regulated by TGF-β promotes hepatocellular carcinoma metastasis.TGF-β 调控的微小肽 JunBP 促进肝癌转移。
Oncogene. 2023 Jan;42(2):113-123. doi: 10.1038/s41388-022-02518-0. Epub 2022 Nov 15.
8
ALKBH5-mediated mA modification of lincRNA LINC02551 enhances the stability of DDX24 to promote hepatocellular carcinoma growth and metastasis.ALKBH5 介导的 lincRNA LINC02551 的 mA 修饰增强了 DDX24 的稳定性，从而促进肝癌的生长和转移。
Cell Death Dis. 2022 Nov 5;13(11):926. doi: 10.1038/s41419-022-05386-4.
9
A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.一种小分子Skp1抑制剂通过p53依赖性机制引发细胞死亡。
iScience. 2022 Jun 14;25(7):104591. doi: 10.1016/j.isci.2022.104591. eCollection 2022 Jul 15.
10
Comprehensive Analysis of Pyroptosis-Related Long Noncoding RNA Immune Infiltration and Prediction of Prognosis in Patients with Colon Cancer.结肠癌患者中焦亡相关长链非编码RNA免疫浸润的综合分析及预后预测
J Oncol. 2022 Jan 18;2022:2035808. doi: 10.1155/2022/2035808. eCollection 2022.
Exp Mol Med. 2020 Oct;52(10):1637-1651. doi: 10.1038/s12276-020-00508-4. Epub 2020 Oct 2.
4
Microenvironment characterization and multi-omics signatures related to prognosis and immunotherapy response of hepatocellular carcinoma.与肝细胞癌预后和免疫治疗反应相关的微环境特征及多组学特征
Exp Hematol Oncol. 2020 May 25;9:10. doi: 10.1186/s40164-020-00165-3. eCollection 2020.
5
SKP1 drives the prophase I to metaphase I transition during male meiosis.SKP1 驱动雄性减数分裂中从前期 I 向中期 I 的转变。
Sci Adv. 2020 Mar 25;6(13):eaaz2129. doi: 10.1126/sciadv.aaz2129. eCollection 2020 Mar.
6
LncRNA PCNAP1 modulates hepatitis B virus replication and enhances tumor growth of liver cancer.长链非编码 RNA PCNAP1 调节乙型肝炎病毒复制并增强肝癌的肿瘤生长。
Theranostics. 2019 Jul 9;9(18):5227-5245. doi: 10.7150/thno.34273. eCollection 2019.
7
Roles and Regulation of Long Noncoding RNAs in Hepatocellular Carcinoma.长链非编码 RNA 在肝细胞癌中的作用与调控。
Cancer Res. 2019 Oct 15;79(20):5131-5139. doi: 10.1158/0008-5472.CAN-19-0255. Epub 2019 Jul 23.
8
LncRNA AY promotes hepatocellular carcinoma metastasis by stimulating transcription.LncRNA AY 通过刺激转录促进肝癌转移。
Theranostics. 2019 Jun 9;9(15):4421-4436. doi: 10.7150/thno.32854. eCollection 2019.
9
Comprehensive analysis of a ceRNA network reveals potential prognostic cytoplasmic lncRNAs involved in HCC progression.综合分析 ceRNA 网络揭示了 HCC 进展中潜在的预后细胞质长非编码 RNA。
J Cell Physiol. 2019 Aug;234(10):18837-18848. doi: 10.1002/jcp.28522. Epub 2019 Mar 27.
10
A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis.一种新型长链非编码 RNA MCM3AP-AS1 通过靶向 miR-194-5p/FOXA1 轴促进肝癌的生长。
Mol Cancer. 2019 Feb 19;18(1):28. doi: 10.1186/s12943-019-0957-7.