Justino Larissa, Baptista Ana A S, Souza Marielen de, Menck-Costa Maísa F, Pires Bárbara G, Cicero Claudineia E, Bracarense Ana P F R L, Kaneko Vanessa M, Oba Alexandre, Okamoto Adriano S, Andreatti Filho Raphael L
Laboratory of Ornithopathology, Department of the of Veterinary Clinic, Center for Animal Pathology, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, Brazil.
Laboratory of Avian Medicine, Department of Preventive Veterinary Medicine, Center for Agrarian Sciences, State University of Londrina, Londrina 86057-970, Brazil.
Animals (Basel). 2022 Jul 22;12(15):1880. doi: 10.3390/ani12151880.
is the etiological agent of NE, a disease that greatly affects the poultry industry. Experiments on the induction of NE are difficult to carry out, as it is a multifactorial disease, and thus different predisposing factors have been used. This study evaluated the effect of the Gumboro disease vaccine virus vaccine (IBDV-vac) associated or not with infection by spp. in broilers, as a predisposing factor for NE. Broilers ( = 99) were divided into groups (11) challenged with IBDV-vac, spp. CP type G (CP13, CP14 and CP03) or both. The macroscopic evaluation revealed that the highest average (3.45) of injury occurred for the CP13 + IBDV-vac group. The microscopic analysis showed that spp. increased the population of intraepithelial lymphocytes and reduced the villus/crypt ratio in duodenum and jejunum when associated with CP13 or CP14. There was a synergistic effect between the CP strain used and the predisposing factors; nevertheless, it was not clear which was the most effective predisposing factor to potentiate the lesions, suggesting that the association of the strain with the factors should first be evaluated for each experimental protocol.
是新城疫的病原体，新城疫是一种对家禽业有重大影响的疾病。由于新城疫是一种多因素疾病，因此进行新城疫诱导实验很困难，所以使用了不同的诱发因素。本研究评估了传染性法氏囊病疫苗病毒疫苗（IBDV-vac）与感染 spp. 相关或不相关对肉鸡新城疫的诱发作用。将99只肉鸡分为11组，分别用IBDV-vac、 spp. CP型G（CP13、CP14和CP03）或两者进行攻毒。宏观评估显示，CP13 + IBDV-vac组的平均损伤最高（3.45）。微观分析表明，当与CP13或CP14联合使用时， spp. 会增加十二指肠和空肠上皮内淋巴细胞数量，并降低绒毛/隐窝比值。所用的CP菌株与诱发因素之间存在协同作用；然而，尚不清楚哪种诱发因素最有效地加剧病变，这表明对于每个实验方案，应首先评估菌株与因素的组合。
