MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Mar Drugs. 2022 Jul 24;20(8):472. doi: 10.3390/md20080472.
Filter-feeding bivalves can accumulate paralytic shellfish toxins (PST) produced by toxic microalgae, which may induce oxidative stress and lipid peroxidation. Peroxisomal acyl-coenzyme A oxidases (ACOXs) are key enzymes functioning in maintaining redox and lipid homeostasis, but their roles in PST response in bivalves are less understood. Herein, a total of six and six s were identified in the and genome, respectively, and the expansion of s was observed. Gene expression analysis revealed an organ/tissue-specific expression pattern in both scallops, with all s being predominantly expressed in the two most toxic organs, digestive glands and kidneys. The regulation patterns of scallop s after exposure to different PST-producing algaes (ACDH) and (AM-1) were revealed. After ACDH exposure, more differentially expressed genes (DEGs) were identified in . digestive glands (three) and kidneys (five) than that in . (two), but the up-regulated DEGs showed similar expression patterns in both species. In . , three DEGs were found in both digestive glands and kidneys after AM-1 exposure, with two same s being acutely and chronically induced, respectively. Notably, these two s also showed different expression patterns in kidneys between ACDH (acute response) and AM-1 (chronic response) exposure. Moreover, inductive expression of s after AM-1 exposure was observed in gills and mantles, and all DEGs in both tissues were up-regulated and their common DEGs exhibited both acute and chronic induction. These results indicate the involvement of scallop s in PST response, and their plasticity expression patterns between scallop species, among tissues, and between the exposure of different PST analogs.
滤食性双壳贝类会积累产毒微藻产生的麻痹性贝类毒素（PST），这可能会引起氧化应激和脂质过氧化。过氧化物酶体酰基辅酶 A 氧化酶（ACOXs）是维持氧化还原和脂质动态平衡的关键酶，但它们在贝类 PST 反应中的作用知之甚少。在此，共在 和 基因组中鉴定出 6 个和 6 个 s，观察到 s 的扩张。基因表达分析显示，两种扇贝的器官/组织具有特异性表达模式，所有 s 主要在两个毒性最大的器官（消化腺和肾脏）中表达。揭示了双壳贝类在暴露于不同 PST 产藻 （ACDH）和 （AM-1）后的 s 调控模式。暴露于 ACDH 后， 消化腺（3 个）和肾脏（5 个）中差异表达基因（DEGs）的数量多于 （2 个），但两种物种中上调的 DEGs 表现出相似的表达模式。在 中，暴露于 AM-1 后，在消化腺和肾脏中发现了 3 个 DEGs，其中 2 个相同的 s 被急性和慢性诱导。值得注意的是，这两个 s 在 ACDH（急性反应）和 AM-1（慢性反应）暴露的肾脏中也表现出不同的表达模式。此外，在暴露于 AM-1 后，s 在鳃和套膜中也表现出诱导表达，并且这两种组织中的所有 DEGs 均上调，其共同的 DEGs 同时表现出急性和慢性诱导。这些结果表明，扇贝 s 参与了 PST 反应，并且它们在扇贝物种之间、组织之间以及不同 PST 类似物暴露之间具有可塑性的表达模式。
