Abidizadegan Maryam, Blomster Jaanika, Fewer David, Peltomaa Elina
Microbiology and Biotechnology Programme, Environmental Laboratory, Faculty of Biological and Environmental Sciences, University of Helsinki, 15140 Lahti, Finland.
Ecosystem and Environment Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, 00014 Helsinki, Finland.
Biology (Basel). 2022 Jul 26;11(8):1112. doi: 10.3390/biology11081112.
The accumulation and production of biochemical compounds in microalgae are influenced by available light quality and algal species-specific features. In this study, four freshwater cryptophyte strains (Cryptomonas ozolinii, C. pyrenoidifera, C. curvata, and C. sp. (CPCC 336)) and one marine strain (Rhodomonas salina) were cultivated under white (control), blue, and green (experimental conditions) lights. Species-specific responses to light quality were detected, i.e., the color of light significantly affected cryptophyte biomass productivity and biochemical compositions, but the optimal light for the highest chemical composition with high antioxidant capacity was different for each algal strain. Overall, the highest phycoerythrin (PE) content (345 mg g−1 dry weight; DW) was reached by C. pyrenoidifera under green light. The highest phenolic (PC) contents (74, 69, and 66 mg g−1 DW) were detected in C. curvata under control conditions, in C. pyrenoidifera under green light, and in C. ozolinii under blue light, respectively. The highest exopolysaccharide (EPS) content (452 mg g−1 DW) was found in C. curvata under the control light. In terms of antioxidant activity, the biochemical compounds from the studied cryptophytes were highly active, with IC50 -values < 50 µg mL−1. Thus, in comparison to well-known commercial microalgal species, cryptophytes could be considered a possible candidate for producing beneficial biochemical compounds.
微藻中生化化合物的积累和产生受可用光质和藻类物种特异性特征的影响。在本研究中，四种淡水隐藻菌株（奥佐利隐藻、具核隐藻、弯隐藻和隐藻属（CPCC 336））和一种海洋菌株（盐生红藻）在白色（对照）、蓝色和绿色（实验条件）光下培养。检测到了物种对光质的特异性反应，即光的颜色显著影响隐藻生物量生产力和生化组成，但对于每种藻类菌株而言，具有高抗氧化能力的最高化学成分的最佳光不同。总体而言，具核隐藻在绿光下达到了最高的藻红蛋白（PE）含量（345 mg g−1干重；DW）。在对照条件下，弯隐藻中检测到最高的酚类（PC）含量（74、69和66 mg g−1 DW），在绿光下具核隐藻中检测到最高的酚类含量，在蓝光下奥佐利隐藻中检测到最高的酚类含量。在对照光下，弯隐藻中发现了最高的胞外多糖（EPS）含量（452 mg g−1 DW）。就抗氧化活性而言，所研究的隐藻中的生化化合物具有高活性，IC50值<50 µg mL−1。因此，与著名的商业微藻物种相比，隐藻可被视为生产有益生化化合物的可能候选者。
