Katyal Gunjan, Ebanks Brad, Dowle Adam, Shephard Freya, Papetti Chiara, Lucassen Magnus, Chakrabarti Lisa
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UK.
Department of Biology, Bioscience Technology Facility, University of York, York YO10 5DD, UK.
Biology (Basel). 2022 Jul 26;11(8):1118. doi: 10.3390/biology11081118.
Antarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mitochondrial proteomes of both the red and white muscle of two different icefish species ( and and compared these with two related red-blooded Notothenioids (, ). Liquid Chromatography-Mass spectrometry (LC-MS/MS) was used to generate and examine the proteomic profiles of the two groups. We recorded a total of 91 differentially expressed proteins in the icefish red muscle mitochondria and 89 in the white muscle mitochondria when compared with the red-blooded related species. The icefish have a relatively higher abundance of proteins involved with Complex V of oxidative phosphorylation, RNA metabolism, and homeostasis, and fewer proteins for striated muscle contraction, haem, iron, creatine, and carbohydrate metabolism. Enrichment analyses showed that many important pathways were different in both red muscle and white muscle, including the citric acid cycle, ribosome machinery and fatty acid degradation. Life in the Antarctic waters poses extra challenges to the organisms that reside within them. Icefish have successfully inhabited this environment and we surmise that species without haemoglobin uniquely maintain their physiology. Our study highlights the mitochondrial protein pathway differences between similar fish species according to their specific tissue oxygenation idiosyncrasies.
南极冰鱼在没有血红蛋白的情况下仍能茁壮成长，这一能力非同寻常。我们想要了解线粒体蛋白质组是如何适应这种蛋白质缺失的。在这些物种中，最有可能重新排列利用氧气的代谢途径。在这里，我们定义了两种不同冰鱼物种（以及）的红色和白色肌肉的线粒体蛋白质组，并将其与两种相关的有红血的南极鱼（，）进行比较。使用液相色谱 - 质谱联用（LC-MS/MS）来生成和检查这两组的蛋白质组图谱。与有红血的相关物种相比，我们在冰鱼红色肌肉线粒体中总共记录到91种差异表达蛋白，在白色肌肉线粒体中记录到89种。冰鱼中参与氧化磷酸化复合体V、RNA代谢和体内平衡的蛋白质丰度相对较高，而参与横纹肌收缩、血红素、铁、肌酸和碳水化合物代谢的蛋白质较少。富集分析表明，红色肌肉和白色肌肉中的许多重要途径都不同，包括柠檬酸循环、核糖体机制和脂肪酸降解。南极水域的生存环境给生活在其中的生物带来了额外的挑战。冰鱼成功地栖息在这个环境中，我们推测没有血红蛋白的物种独特地维持着它们的生理机能。我们的研究突出了相似鱼类物种之间根据其特定组织氧合特性而存在的线粒体蛋白质途径差异。
