Cooper Robin Lewis, Thomas Matthew A, McLetchie David Nicholas
Department of Biology, University of Kentucky, Lexington, KY 40506-0225, USA.
Methods Protoc. 2022 Jun 30;5(4):56. doi: 10.3390/mps5040056.
Electrical activity is widely used for assessing a plant's response to an injury or environmental stimulus. Commonly, a differential electrode recording between silver wire leads with the reference wire connected to the soil, or a part of the plant, is used. One method uses KCl-filled glass electrodes placed into the plant, similar to recording membrane/cell potentials in animal tissues. This method is more susceptible to artifacts of equipment noise and photoelectric effects than an impedance measure. An impedance measure using stainless steel wires is not as susceptible to electrically induced noises. Impedance measurements are able to detect injury in plants as well as exposure of the roots to environmental compounds (glutamate). The impedance measures were performed in 5 different plants (tomato, eggplant, pepper, liverwort, and ), and responses to mechanical movement of the plant, as well as injury, were recorded. Monitoring electrical activity in a plant that arises in a distant plant was also demonstrated using the impedance method. The purpose of this report is to illustrate the ease in using impedance measures for monitoring electrical signals from individual plants or aggregates of plants for potentially scaling for high throughput and monitoring controlled culturing and outdoor field environments.
电活动被广泛用于评估植物对损伤或环境刺激的反应。通常，使用连接到土壤或植物一部分的银丝引线之间的差分电极记录，其中参考线连接到土壤或植物的一部分。一种方法是将填充KCl的玻璃电极插入植物中，类似于在动物组织中记录膜/细胞电位。与阻抗测量相比，这种方法更容易受到设备噪声和光电效应的伪影影响。使用不锈钢丝的阻抗测量对电感应噪声的敏感度较低。阻抗测量能够检测植物中的损伤以及根部对环境化合物（谷氨酸）的暴露。在5种不同的植物（番茄、茄子、辣椒、地钱等）中进行了阻抗测量，并记录了植物对机械运动以及损伤的反应。还使用阻抗方法证明了监测远处植物中产生的电活动。本报告的目的是说明使用阻抗测量来监测来自单个植物或植物聚集体的电信号的简便性，以便有可能扩大规模用于高通量以及监测受控培养和户外田间环境。
