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评估商用紫外线设备对塑料表面微生物灭活效果的方案

Protocol for Evaluating the Microbial Inactivation of Commercial UV Devices on Plastic Surfaces.

作者信息

Haley Olivia C, Zhao Yeqi, Bhullar Manreet

机构信息

Department of Horticulture and Natural Resources, Kansas State University, Olathe, KS 66061, USA.

Food Science Institute, Kansas State University, Manhattan, KS 66506, USA.

出版信息

Methods Protoc. 2022 Jul 22;5(4):65. doi: 10.3390/mps5040065.

DOI:10.3390/mps5040065
PMID:35893591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9332507/
Abstract

With the plethora of commercially available UV-C devices exhibiting different intensity and lifespans, it is critical to consumer safety that companies verify and clearly communicate the efficacy of their devices as per the intended use. The purpose of this study was to define a low-cost protocol for investigating the antimicrobial efficacy of commercial UV devices for industry use. The tested devices included: a wall-mounted unit (Device A), a troffer unit (Device B), and an induction lamp unit (Device C). The devices were installed within an enclosed tower to prevent the transmission of UV-C radiation outside of the testing area. The procedure details determining the devices' antimicrobial efficacy using plastic coupons inoculated with or The protocol includes suggested time-distance treatments according to the potential application of each device type and reports the results as log CFU/mL reduction or percent reduction.

摘要

由于市面上有大量不同强度和使用寿命的紫外线C设备,各公司按照预期用途验证并清晰传达其设备的功效对消费者安全至关重要。本研究的目的是定义一种低成本方案,用于调查工业用商用紫外线设备的抗菌功效。测试的设备包括:一个壁挂式装置(设备A)、一个吸顶式装置(设备B)和一个感应灯装置(设备C)。这些设备安装在一个封闭的塔内,以防止紫外线C辐射传播到测试区域之外。该程序详细说明了使用接种了……或……的塑料试片来确定设备抗菌功效的方法。该方案包括根据每种设备类型的潜在应用建议的时间-距离处理,并将结果报告为每毫升菌落形成单位(CFU/mL)减少对数或减少百分比。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a929/9332507/f25e81b542c1/mps-05-00065-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a929/9332507/d726ce865ecc/mps-05-00065-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a929/9332507/f25e81b542c1/mps-05-00065-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a929/9332507/d726ce865ecc/mps-05-00065-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a929/9332507/f25e81b542c1/mps-05-00065-g002.jpg

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本文引用的文献

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Hot, Humid Air Decontamination of Aircraft Confirmed That High Temperature and High Humidity Are Critical for Inactivation of Infectious, Enveloped Ribonucleic Acid (RNA) Virus.飞机的热湿空气净化证实,高温和高湿对于灭活有传染性的包膜核糖核酸(RNA)病毒至关重要。
Front Bioeng Biotechnol. 2020 Oct 23;8:592621. doi: 10.3389/fbioe.2020.592621. eCollection 2020.
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Ultraviolet irradiation doses for coronavirus inactivation - review and analysis of coronavirus photoinactivation studies.用于冠状病毒灭活的紫外线照射剂量——冠状病毒光灭活研究的综述与分析
GMS Hyg Infect Control. 2020 May 14;15:Doc08. doi: 10.3205/dgkh000343. eCollection 2020.
3
A chromatin scaffold for DNA damage recognition: how histone methyltransferases prime nucleosomes for repair of ultraviolet light-induced lesions.
一种用于 DNA 损伤识别的染色质支架:组蛋白甲基转移酶如何为修复紫外线诱导损伤的核小体做准备。
Nucleic Acids Res. 2020 Feb 28;48(4):1652-1668. doi: 10.1093/nar/gkz1229.
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Ultraviolet-C decontamination of a hospital room: Amount of UV light needed.医院病房的紫外线C消毒:所需紫外线量
Burns. 2020 Jun;46(4):842-849. doi: 10.1016/j.burns.2019.10.004. Epub 2019 Oct 30.
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Ultraviolet A and B wavelength-dependent inactivation of viruses and bacteria in the water.水中病毒和细菌的紫外线A和B波长依赖性灭活
J Water Health. 2018 Oct;16(5):796-806. doi: 10.2166/wh.2018.071.
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Efficacy of UV light treatment for the microbiological decontamination of chicken, associated packaging, and contact surfaces.紫外线处理对鸡肉、相关包装和接触表面的微生物消毒效果。
J Food Prot. 2011 Apr;74(4):565-72. doi: 10.4315/0362-028X.JFP-10-356.
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Effect of Ultra-violet Irradiation of Classrooms on Spread of Measles in Large Rural Central Schools Preliminary Report.农村大型中心校教室紫外线照射对麻疹传播的影响初步报告
Am J Public Health Nations Health. 1947 May;37(5):529-37.
8
Comparison of UV C light and chemicals for disinfection of surfaces in hospital isolation units.医院隔离病房中紫外线C光与化学消毒剂对物体表面消毒效果的比较
Infect Control Hosp Epidemiol. 2006 Jul;27(7):729-34. doi: 10.1086/503643. Epub 2006 Jun 2.
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Proc Natl Acad Sci U S A. 1964 Feb;51(2):293-300. doi: 10.1073/pnas.51.2.293.
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Individual determination of the yield of the main UV-induced dimeric pyrimidine photoproducts in DNA suggests a high mutagenicity of CC photolesions.对DNA中主要紫外线诱导的二聚嘧啶光产物产量的单独测定表明CC光损伤具有高致突变性。
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